Leung K N, Ada G L
Scand J Immunol. 1980;12(5):393-400. doi: 10.1111/j.1365-3083.1980.tb00083.x.
Effector cells that demonstrate delayed-type hypersensitivity (DTH) on transfer with antigen to naive mice can be recovered from the lungs of mice inoculated intranasally 6 days earlier with a lethal dose (usually 5x10(1)EID50) of influenza A virus. The activity recovered was proportional to the dose of virus instilled intranasally and the extent of lung consolidation observed. Active cells could also be recovered from the draining lymph nodes and from the peripheral blood. The effector cells were identified as T lymphocytes of Ly 1 phenotype and required I-region sharing between donor and recipient for activity to be elicited. They were cross-reactive within the A group of influenza viruses. Two experiments are reported in which immune cell preparations that expressed DTH activity but had very little cytotoxic T cell activity were transferred to mice inoculated 1 or 2 days earlier with a lethal dose of virus. The mice were not protected from death, and in both experiments, the recipient mice died more rapidly than the controls. These results contrast with earlier results in which cell preparations with high cytotoxic T-cell activity were shown to protect recipient infected mice from death.
将抗原转移至未致敏小鼠时表现出迟发型超敏反应（DTH）的效应细胞，可从6天前经鼻接种致死剂量（通常为5×10¹EID50）甲型流感病毒的小鼠肺中分离得到。所恢复的活性与经鼻滴注的病毒剂量以及观察到的肺实变程度成正比。活性细胞也可从引流淋巴结和外周血中分离得到。效应细胞被鉴定为Ly 1表型的T淋巴细胞，且供体和受体之间需要I区共享才能引发活性。它们在甲型流感病毒A组内具有交叉反应性。报道了两个实验，其中将表达DTH活性但细胞毒性T细胞活性极低的免疫细胞制剂转移至1或2天前接种致死剂量病毒的小鼠体内。小鼠未得到死亡保护，且在两个实验中，受体小鼠比对照组死亡更快。这些结果与早期结果形成对比，早期结果显示具有高细胞毒性T细胞活性的细胞制剂可保护受感染的受体小鼠免于死亡。
