Leung K N, Ada G L
J Exp Med. 1981 May 1;153(5):1029-43. doi: 10.1084/jem.153.5.1029.
Injection of mice with infectious or noninfectious preparations of influenza virus induces the formation of T cells which, when added to primary tissue cultures of normal spleen cells exposed to influenza virus, enhance the generation of effector T cells which mediate delayed-type hypersensitivity (DTH) reaction. The enhancing cells possess Thy-1 and Ly-1 surface antigens are radioresistant and antigen-specific. If infectious virus was used to stimulate the DTH response in vitro, help was delivered whether homologous or heterologous A strain influenza virus was used to generate the helper T cells (Th) in vivo. In contrast, only Th cells generated using homologous virus were effective if noninfectious virus was used to stimulate the DTH response in vitro. Peak helper activity occurred 2 d after virus injection and the Th cells were only effective if added to the primary cultures within 24 h after addition of the stimulating antigen. The Th cells enhanced the generation of both classes of DTH effector cells, i.e., those that are Ly-1 positive and IA-subregion restricted and those that are Ly-2,3 positive and K,D-region restricted. The activity of the Th cells was found to be IA-subregion restricted and this was shown to operate at the level of the stimulator cells so that the delivery of help to the responder cells was not H-2 restricted. The possibility that the Th cells might be a precursor to the Ly-1 positive IA subregion-restricted DTH effector cells is discussed.
给小鼠注射传染性或非传染性流感病毒制剂可诱导T细胞形成，将这些T细胞添加到暴露于流感病毒的正常脾细胞原代组织培养物中时，可增强介导迟发型超敏反应（DTH）的效应T细胞的生成。增强细胞具有Thy-1和Ly-1表面抗原，具有辐射抗性且具有抗原特异性。如果在体外使用传染性病毒刺激DTH反应，无论在体内使用同源还是异源A株流感病毒来产生辅助性T细胞（Th），都能提供帮助。相反，如果在体外使用非传染性病毒刺激DTH反应，只有使用同源病毒产生的Th细胞才有效。辅助活性在病毒注射后2天达到峰值，并且Th细胞只有在添加刺激抗原后24小时内添加到原代培养物中才有效。Th细胞增强了两类DTH效应细胞的生成，即Ly-1阳性且IA亚区受限的细胞以及Ly-2、3阳性且K、D区受限的细胞。发现Th细胞的活性受IA亚区限制，并且这在刺激细胞水平起作用，因此向反应细胞提供帮助不受H-2限制。本文讨论了Th细胞可能是Ly-1阳性IA亚区受限的DTH效应细胞前体的可能性。
