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[碳水化合物试验对葡萄球菌种间鉴别诊断的重要性]

[Importance of carbohydrate tests for interspecies differentiation of staphylococci].

作者信息

Safonova T B, Shcherbakova N A, Afanas'eva T I, Sobolev V R

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1978 Sep(9):98-101.

PMID:747032
Abstract

The authors present the results of interspecies identification of coagulaso-negative staphylococci isolated from different sources. Modification of the method of aerobic carbohydrate and alcohols fermentation of hard media is described. Use of the latest recommendations of the International Subcommittee on taxonomy of staphylocossi and micrococci permitted to identify the greater part of 120 strains studied as Staph. epidermidis and Staph. saprophyticus; however, 1/3 of the cultures differed from these species ("intermediate" strains). Galactose and mannose fermentation was characteristic of Staph. epidermidis, and the absence of fermentation of these carbohydrates--of Staph. saprophyticus. The differences in the fermentation of mannite and tregalose, on the one hand, and on mannose and galactose--on the other, were characteristic of 57% of the "intermediate" strains. The data obtained permitted to draw a conclusion on nonhomogeneity of the group of coagulase-negative staphylococci by their reference to different carbohydrates and alcohols, and on a possibility of existence of not only of different species of these staphylococci, but also of their biochemical variants.

摘要

作者展示了从不同来源分离出的凝固酶阴性葡萄球菌的种间鉴定结果。描述了对硬培养基需氧碳水化合物和醇类发酵方法的改进。采用葡萄球菌和微球菌分类国际小组委员会的最新建议,得以将所研究的120株菌株中的大部分鉴定为表皮葡萄球菌和腐生葡萄球菌;然而,1/3的培养物与这些菌种不同(“中间”菌株)。半乳糖和甘露糖发酵是表皮葡萄球菌的特征,而这些碳水化合物不发酵则是腐生葡萄球菌的特征。一方面,甘露醇和海藻糖发酵的差异,另一方面,甘露糖和半乳糖发酵的差异,是57%的“中间”菌株的特征。所获得的数据使得能够得出结论,即凝固酶阴性葡萄球菌组在涉及不同碳水化合物和醇类方面具有非均质性,并且这些葡萄球菌不仅可能存在不同的菌种,还可能存在其生化变体。

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