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一种通过对测试培养基进行多点接种来鉴定葡萄球菌的简化系统。

A simplified system for the identification of staphylococci by multipoint inoculation of test media.

作者信息

Reuther J W

出版信息

J Med Microbiol. 1986 Sep;22(2):179-82. doi: 10.1099/00222615-22-2-179.

DOI:10.1099/00222615-22-2-179
PMID:3528500
Abstract

Most hospital bacteriologists have divided staphylococci into two groups: Staphylococcus aureus and the coagulase-negative staphylococci of which the novobiocin-resistant varieties are termed S. saprophyticus. The identification of S. aureus has been easy but that of the other staphylococci has provided some difficulties and most currently available methods are expensive or time consuming. Multipoint inoculation of a set of test media provides a convenient way of identifying large numbers of staphylococcal isolates. In tests with 118 isolates, mainly clinical but including some environmental isolates and some from the National Collection of Type Cultures, there was 90.7% agreement between identifications by the API-Staph system and by the multipoint system. The remaining 9.3% of strains was identified by the multipoint system but could not be identified by use of the data supplied in the API-Staph kit.

摘要

大多数医院细菌学家将葡萄球菌分为两类

金黄色葡萄球菌和凝固酶阴性葡萄球菌,其中对新生霉素耐药的变种被称为腐生葡萄球菌。金黄色葡萄球菌的鉴定很容易,但其他葡萄球菌的鉴定存在一些困难,而且目前大多数可用方法都昂贵或耗时。在一组测试培养基上进行多点接种为鉴定大量葡萄球菌分离株提供了一种便捷的方法。在用118株分离株进行的测试中,主要是临床分离株,但包括一些环境分离株和一些来自国家典型培养物保藏中心的菌株,API葡萄球菌系统鉴定结果与多点系统鉴定结果之间的一致性为90.7%。其余9.3%的菌株由多点系统鉴定,但无法根据API葡萄球菌试剂盒提供的数据进行鉴定。

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