Fushitani S, Minakuchi K, Tsuchiya K, Takasugi M, Murakami K
Department of Pharmacy, Tokushima University Hospital, Japan.
Yakugaku Zasshi. 1995 Aug;115(8):611-7. doi: 10.1248/yakushi1947.115.8_611.
It is thought that highly reactive oxygen radicals generated at the ischemia-reperfusion in case of strokes play an important role in damaging the brain. It is well known that lipid peroxidation is propagated by active oxygen radicals, and the the brain is susceptible to the lipid peroxidation. In the previous study, we found that several Chinese herbal medicines and kampo components, which were used for the attenuation of the post-ischemic brain injury, showed a free-radical (OH., O2-. and DPPH) scavenging activity. However, it is not clear whether these Chinese herbal medicines can inhibit the lipid peroxidation reaction or not. In attempting to address this question, we have used three kinds of kampo formulations (Oren-gedoku-to (Huang-Lian-Jie-Du-Tang), Saiko-ka-ryukotsu-borei-to (Chai-Hu-Jia-Long-Gu-Mu-Li-Tang) and Keishi-bukuryo-gan (Gui-Zhi-Fu-Ling-Wan)) to measure the suppressive effect of the lipid peroxidation on the mouse cerebrum using the TBA technique in vitro and in vivo. In vitro experiments, all these Chinese herbal medicines decreased the levels of TBA-reactive substances concentration-dependently. In vivo studies, the levels of the TBA-reactive substance of the cerebrum homogenate of mice treated with these kampo formulations by p.o. for three weeks also decreased. From these results, we suggest that the pharmacological action of Chinese herbal medicines used for the attenuation of the post-ischemic brain damage not only have a free-radical scavenging activity, but also have a suppressive effect on the generation of the lipid peroxidation.
人们认为，中风时缺血再灌注产生的高活性氧自由基在损伤大脑方面起着重要作用。众所周知，活性氧自由基会引发脂质过氧化，而大脑易受脂质过氧化影响。在先前的研究中，我们发现几种用于减轻缺血后脑损伤的中草药和汉方成分具有清除自由基（OH·、O₂⁻和DPPH）的活性。然而，这些中草药是否能抑制脂质过氧化反应尚不清楚。为了解决这个问题，我们使用了三种汉方制剂（黄连解毒汤、柴胡加龙骨牡蛎汤和桂枝茯苓丸），通过TBA技术在体外和体内测量其对小鼠大脑脂质过氧化的抑制作用。在体外实验中，所有这些中草药均浓度依赖性地降低了TBA反应性物质的水平。在体内研究中，经口给予这些汉方制剂三周的小鼠大脑匀浆中TBA反应性物质的水平也有所降低。从这些结果来看，我们认为用于减轻缺血后脑损伤的中草药的药理作用不仅具有清除自由基的活性，而且对脂质过氧化的产生也有抑制作用。
