Nishimura T, Kometani T, Okada S, Ueno N, Yamamoto T
Biochemical Research Laboratory, Ezaki Glico Co. Ltd., Osaka, Japan.
Yakugaku Zasshi. 1995 Aug;115(8):626-32. doi: 10.1248/yakushi1947.115.8_626.
Inhibitory effects of hydroquinone-alpha-glucoside (HQ-alpha-G) on the melanogenesis were investigated and compared with those of arbutin. The levels of inhibitory effects of HQ-alpha-G and arbutin on the tyrosinase activity were nearly the same. Inhibitory effects of both compounds on the melanogenesis, were studied using cultured B16 melanoma cells, and HQ-alpha-G was also found to have a similar effect to that of arbutin without inhibiting cell growth. In this experiment, while HQ-alpha-G hardly inhibited cell growth at 1 mM, arbutin inhibit it significantly at the same concentration. From these results it is suggested that HQ-alpha-G as well as arbutin inhibited the melanogenesis by affecting tyrosinase rather than by killing melanocytes. Furthermore, the melanogenesis of guinea-pigs with brown hair was reduced to about 80% by applying them each compound. The great differences in toxicity to normal human keratinocyte were not recognized between these two glucosides. It is, therefore, considered that HQ-alpha-G is an effective and safe ingredient for cosmetics.
研究了对苯二酚-α-葡萄糖苷（HQ-α-G）对黑色素生成的抑制作用，并与熊果苷的抑制作用进行了比较。HQ-α-G和熊果苷对酪氨酸酶活性的抑制水平几乎相同。使用培养的B16黑色素瘤细胞研究了这两种化合物对黑色素生成的抑制作用，发现HQ-α-G也具有与熊果苷类似的作用，且不抑制细胞生长。在本实验中，1 mM的HQ-α-G几乎不抑制细胞生长，而相同浓度的熊果苷则显著抑制细胞生长。从这些结果表明，HQ-α-G和熊果苷通过影响酪氨酸酶而不是杀死黑素细胞来抑制黑色素生成。此外，通过涂抹每种化合物，棕色毛豚鼠的黑色素生成减少到约80%。这两种糖苷对正常人角质形成细胞的毒性没有很大差异。因此，认为HQ-α-G是一种有效且安全的化妆品成分。
