Yamaoka K, Kanbe M
Department of Laboratory Medicine, Hiroshima University School of Medicine.
Rinsho Byori. 1995 Aug;43(8):799-804.
The gender verification test (GV) was carried out by gene diagnostics at the 12th Asian Games in Hiroshima, 1994. Human genomic DNA samples were extracted from the hair root of female competitors. Polymerase chain reaction (PCR) was used to prove the absence of the sex determining region of Y-chromosome (SRY) in order to confirm the femininity, and detection of pseudo autosomal boundary region of Y-chromosome (PABY) in order to confirm that the DNA is extracted from the hair root and the femininity can again be confirmed. A total of 1,300 female competitors were screened. We conclude that this testing is useful for the GV, because hair sampling is easy, the method is simple and there is little contamination.
1994年在广岛举行的第12届亚运会期间，通过基因诊断进行了性别验证测试（GV）。从女性参赛者的发根中提取人类基因组DNA样本。采用聚合酶链反应（PCR）来证明Y染色体性别决定区（SRY）不存在，以确认其女性身份；同时检测Y染色体假常染色体边界区域（PABY），以确认DNA是从发根中提取的，并且可以再次确认其女性身份。总共对1300名女性参赛者进行了筛查。我们得出结论，这种检测方法对性别验证测试很有用，因为毛发采样容易，方法简单，且污染少。
