Tombes R M
Massey Cancer Center, Medical College of Virginia, Richmond 23298, USA.
Methods Cell Biol. 1995;47:467-72. doi: 10.1016/s0091-679x(08)60846-8.
Phosphagen kinases play a role in metabolic function in diverse species (Bessman and Carpenter, 1985). Enzyme assays and inhibitors have been used successfully to demonstrate the role of one of these enzyme families in sea urchins. Although arginine kinase was not purified, its enzymatic activity has been detected in sea urchin eggs and embryos and many other species by simply substituting phosphoarginine (PArg) for PCr (Fujimaki and Yanagisawa, 1978; Tombes and Shapiro, 1989). ArgK does not show sensitivity to FDNB, but CrK proteins of the appropriate molecular weight were detected with this reagent in cells from many species (Tombes and Shapiro, 1989). The use of these techniques to identify and study function of phosphagen kinases in diverse species has much potential.
磷酸原激酶在多种物种的代谢功能中发挥作用（贝斯曼和卡彭特，1985年）。酶分析和抑制剂已成功用于证明这些酶家族之一在海胆中的作用。尽管精氨酸激酶未被纯化，但通过简单地用磷酸精氨酸（PArg）替代磷酸肌酸（PCr），已在海胆卵和胚胎以及许多其他物种中检测到其酶活性（藤牧和柳泽，1978年；汤姆斯和夏皮罗，1989年）。精氨酸激酶对2,4-二硝基氟苯（FDNB）不敏感，但用该试剂在许多物种的细胞中检测到了适当分子量的肌酸激酶（CrK）蛋白（汤姆斯和夏皮罗，1989年）。利用这些技术鉴定和研究多种物种中磷酸原激酶的功能具有很大潜力。
