Dell'Osso G, Avitabile M, Sciacca G, Lanteri E, Stivala F
Istituto di Medicina Legale e delle Assicurazioni, Università degli Studi, Catania.
Minerva Stomatol. 1995 May;44(5):205-9.
Often teeth are the only items which can be used for personal identification in forensic medicine. In the present work we describe a method to extract and amplify DNA from dental elements ranging from 2 weeks to 5 year from the avulsion. PCR (polymerase chain reaction) was used to amplify VNTR sequences; the alleles products were electrophoresed, visualized by traditional methods and compared to the amplified products obtained from the matching blood sample. Our results give a new and powerful investigative tool for personal identification in the field of forensic odontostomatology, since such a procedure can be successfully applied both to recent and to ancient teeth.
在法医学中，牙齿常常是唯一可用于个人身份鉴定的物品。在本研究中，我们描述了一种从拔牙后2周龄至5岁龄的牙齿样本中提取和扩增DNA的方法。采用聚合酶链反应（PCR）扩增VNTR序列；对等位基因产物进行电泳，通过传统方法显色，并与从匹配血样中获得的扩增产物进行比较。我们的研究结果为法医口腔医学领域的个人身份鉴定提供了一种全新且强大的调查工具，因为这一程序可成功应用于新鲜牙齿和古老牙齿。
