Jones S J, Baillie D L
Institute of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, B.C., Canada.
Mol Gen Genet. 1995 Oct 25;248(6):719-26. doi: 10.1007/BF02191712.
A mutation in the let-653 gene of Caenorhabditis elegans results in larval death. The lethal arrest is concurrent with the appearance of a vacuole anterior to the lower pharyngeal bulb. The position of the vacuole is consistent with a dysfunction of the secretory/excretory apparatus. Germline transformation rescue experiments were able to position the let-653 gene to two overlapping cosmid subclones. Sequence data generated from both cDNA and genomic DNA subclones indicated that let-653 encodes a mucin-like protein. Our characterization suggests that a mucin-like protein is essential for effective functioning of the secretory/excretory apparatus within C. elegans.
秀丽隐杆线虫let-653基因的突变会导致幼虫死亡。致死性发育停滞与下咽球下方出现一个液泡同时发生。液泡的位置与分泌/排泄器官的功能障碍一致。生殖系转化拯救实验能够将let-653基因定位到两个重叠的黏粒亚克隆上。从cDNA和基因组DNA亚克隆生成的序列数据表明，let-653编码一种黏蛋白样蛋白。我们的特征分析表明，黏蛋白样蛋白对于秀丽隐杆线虫体内分泌/排泄器官的有效运作至关重要。
