Gadient R A, Lachmund A, Unsicker K, Otten U
Department of Physiology, University of Basel, Switzerland.
Neurosci Lett. 1995 Jul 14;194(1-2):17-20. doi: 10.1016/0304-3940(95)11708-5.
Using in situ hybridization and reverse transcription combined with the polymerase chain reaction (RT-PCR) we have studied the expression and developmental regulation of interleukin-6 (IL-6) and its receptor mRNAs in rat adrenal medulla. Labeling for both transcripts was much more intense over the adrenal medulla than over cortical regions at postnatal day 70. Levels of mRNAs of both genes measured by RT-PCR increased from birth to adulthood. In vitro survival and neurite growth of chromaffin cells from early postnatal rats were not affected by IL-6. Even so, the presence and developmental regulation of IL-6 and its receptor are consistent with a possible auto- or paracrine role of IL-6 in rat adrenal medulla.
我们运用原位杂交以及逆转录结合聚合酶链反应（RT-PCR）技术，研究了白细胞介素-6（IL-6）及其受体mRNA在大鼠肾上腺髓质中的表达及发育调控情况。在出生后第70天，肾上腺髓质上这两种转录本的标记信号远比皮质区域强烈。通过RT-PCR测定，这两个基因的mRNA水平从出生到成年均有所增加。出生后早期大鼠嗜铬细胞的体外存活及神经突生长不受IL-6影响。即便如此，IL-6及其受体的存在与发育调控情况表明，IL-6在大鼠肾上腺髓质中可能具有自分泌或旁分泌作用。
