Skopek R J, Liljemark W F
Department of Diagnostic and Surgical Sciences, University of Minnesota School of Dentistry, Minneapolis 55455, United States.
Oral Microbiol Immunol. 1994 Feb;9(1):19-24. doi: 10.1111/j.1399-302x.1994.tb00209.x.
The mechanism of bacterial adherence to the mat of bacteria in preformed dental plaque is not well defined. This study measured the influence of saliva on the adherence of bacteria in suspension to a continuous bacterial surface in vitro. Twenty different pairs of bacteria were tested, consisting of Streptococcus spp., Haemophilus spp. and Actinomyces spp. The species were chosen based on the parameters of coaggregation, and salivary agglutination. The results were expressed as bacteria that adhered per mm2 of bacterial surface. When both the surface bacteria and the bacteria in suspension agglutinated in saliva, interbacterial adherence was increased 2.5-fold when a salivary coating was placed on the surface. When one or both of the bacteria did not agglutinate in saliva, interbacterial adherence was increased only slightly when the surface was saliva-coated. The results suggested that salivary-mediated adherence is significant to plaque formation once the tooth surface becomes covered with bacteria. Thus bacteria that are capable of agglutinating in saliva may have a distinct advantage in colonization of the plaque surface.
细菌在预先形成的牙菌斑中附着于细菌生物膜的机制尚未明确。本研究在体外测量了唾液对悬浮细菌附着于连续细菌表面的影响。测试了20对不同的细菌，包括链球菌属、嗜血杆菌属和放线菌属。这些菌种是根据共聚和唾液凝集参数选择的。结果以每平方毫米细菌表面附着的细菌数量表示。当表面细菌和悬浮细菌在唾液中都发生凝集时，在表面涂上唾液涂层后，细菌间的附着增加了2.5倍。当一种或两种细菌在唾液中不发生凝集时，表面涂上唾液涂层后，细菌间的附着仅略有增加。结果表明，一旦牙齿表面被细菌覆盖，唾液介导的附着对牙菌斑形成具有重要意义。因此，能够在唾液中凝集的细菌在菌斑表面定植方面可能具有明显优势。
