Samaranayake L P, Hamilton D, MacFarlane T W
Oral Biology Unit, Faculty of Dentistry, University of Hong Kong.
Oral Microbiol Immunol. 1994 Aug;9(4):236-40. doi: 10.1111/j.1399-302x.1994.tb00064.x.
Despite the numerous investigations on the adhesion of microorganisms to buccal epithelial cells, it is difficult to correlate different results, as disparate adherence values have been reported for a given organism. As one reason for this disparity may be the indigenous or natural bacterial populations on human buccal epithelial cells, the effect of the latter on subsequent microbial adherence in vitro was examined. There was a highly significant correlation between the degree of natural bacterial colonization on pooled buccal epithelial cells from 8 healthy donors and the adhesion of a single isolate each of Streptococcus mitis, Escherichia coli and Actinomyces naeslundii. However, no such relationship could be established for Candida albicans, Streptococcus milleri and another isolate of Streptococcus mitis. As in previous studies, variation in adherence values was found, both between samples from different donors, and from the same donor over time, but to a far lesser degree in pooled samples from different donors. These results imply that natural bacterial populations on buccal epithelial cells may affect the adhesion values derived from laboratory experimentation, and hence such data should be interpreted with caution.
尽管对微生物与颊上皮细胞的黏附进行了大量研究，但由于对于特定生物体报道了不同的黏附值，因此难以将不同的结果相互关联。由于这种差异的一个原因可能是人类颊上皮细胞上的固有或天然细菌群落，因此研究了后者对体外后续微生物黏附的影响。来自8名健康供体的汇集颊上皮细胞上的天然细菌定植程度与缓症链球菌、大肠杆菌和内氏放线菌各单一分离株的黏附之间存在高度显著的相关性。然而，对于白色念珠菌、米勒链球菌和缓症链球菌的另一种分离株，无法建立这样的关系。与之前的研究一样，在来自不同供体的样本之间以及同一供体随时间的样本中，均发现了黏附值的变化，但在来自不同供体的汇集样本中，这种变化程度要小得多。这些结果表明，颊上皮细胞上的天然细菌群落可能会影响实验室实验得出的黏附值，因此对此类数据的解释应谨慎。
