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The purine-rich trinucleotide repeat sequences d(CAG)15 and d(GAC)15 form hairpins.

作者信息

Yu A, Dill J, Mitas M

机构信息

Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078, USA.

出版信息

Nucleic Acids Res. 1995 Oct 25;23(20):4055-7. doi: 10.1093/nar/23.20.4055.

DOI:10.1093/nar/23.20.4055
PMID:7479064
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307342/
Abstract

The structures of single-stranded (ss) oligonucleotides containing (CAG)15 [ss(CAG)15] or (GAC)15 [ss(GAC)15] were examined. At 10 degrees C, the electrophoretic mobilites of the two DNAs were similar to ss(CTG)15, a DNA that forms a hairpin containing base paired and/or stacked thymines. At 37 degrees C in 50 mM NaCl, single-strand-specific P1 nuclease cleaved the G33-G36 phosphodiesters of ss(GAC)15, and the G32-A34, G35-C36 phosphodiesters of ss(CAG)15 (where the loop apex of both DNAs = A34). Electrophoretic mobility melting profiles indicated that the melting temperature (Tm) of ss(CAG)15 in low (approximately 1 mM Na+) ionic strength was 38 degrees C. In contrast, the Tm of ss(GAC)15 was 49 degrees C, a value similar to the Tm of ss(CTG)15. These results provide evidence that ss(GAC)15 and ss(CAG)15 form similar, but distinguishable hairpin structures.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46a2/307342/269fd9661666/nar00020-0052-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46a2/307342/269fd9661666/nar00020-0052-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46a2/307342/269fd9661666/nar00020-0052-a.jpg

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