Fulton R W, Confer A W, Burge L J, Perino L J, d'Offay J M, Payton M E, Mock R E
Department of Veterinary Parasitology, Microbiology, and Public Health, Oklahoma State University, Stillwater 74078, USA.
Vaccine. 1995;13(8):725-33. doi: 10.1016/0264-410x(94)00072-u.
Calves were vaccinated with four different commercial viral vaccines containing bovine herpesvirus-1 (BHV-1), bovine viral diarrhea (BVDV), parainfluenza-3 virus (PI-3V), and bovine respiratory syncytial virus (BRSV) immunogens. For the initial vaccination certain vaccines were given twice (days 0 and 28), whereas other vaccines were given on day 0. The calves received another injection on day 140 with the vaccine originally given on day 0. The sera were collected at days 0, 7, 14, 21, 28, 42, 56, 84, 112, 140, 154, 168, and 196 and assayed for viral neutralizing antibodies. The calves were seronegative to BHV-1, BVDV, and BRSV at the onset of the experiment; however, the calves were PI-3V antibody positive due to prior active infection. The commercial vaccines were: (I) inactivated; (II) modified live virus (MLV); (III) combination of chemically altered live virus, MLV, and inactivated virus; and (IV) combination of inactivated and MLV. Among the vaccine groups there were differences in onset and duration of antibodies as measured by geometric mean titers to each immunogen in postvaccination collection dates compared to day 0 titers; and likewise compared to day 140 titers after revaccination at day 140. There were also differences in antibody titers to the various viruses among the vaccine groups on specific collection dates. All four vaccines induced increased BHV-1 antibodies by day 14 after the initial injection. The antibody titers induced by MLV BHV-1 and the chemically altered BHV-1 vaccines had greater duration than those induced by the inactivated vaccine.(ABSTRACT TRUNCATED AT 250 WORDS)
给犊牛接种了四种不同的商业病毒疫苗，这些疫苗含有牛疱疹病毒1型（BHV - 1）、牛病毒性腹泻病毒（BVDV）、副流感3型病毒（PI - 3V）和牛呼吸道合胞病毒（BRSV）免疫原。对于初次接种，某些疫苗接种两次（第0天和第28天），而其他疫苗在第0天接种。犊牛在第140天用最初在第0天接种的疫苗再次注射。在第0、7、14、21、28、42、56、84、112、140、154、168和196天采集血清，并检测病毒中和抗体。在实验开始时，犊牛对BHV - 1、BVDV和BRSV血清学阴性；然而，由于先前的活跃感染，犊牛PI - 3V抗体呈阳性。商业疫苗有：（I）灭活疫苗；（II）改良活病毒（MLV）疫苗；（III）化学改变的活病毒、MLV和灭活病毒的组合疫苗；（IV）灭活疫苗和MLV的组合疫苗。在疫苗组中，与第0天的滴度相比，在接种后采集日期通过对每种免疫原的几何平均滴度测量的抗体出现时间和持续时间存在差异；同样，与第140天再次接种后第140天的滴度相比也存在差异。在特定采集日期，疫苗组之间对各种病毒的抗体滴度也存在差异。所有四种疫苗在初次注射后第14天诱导BHV - 1抗体增加。MLV BHV - 1疫苗和化学改变的BHV - 1疫苗诱导的抗体滴度持续时间比灭活疫苗诱导的更长。（摘要截短至250字）
