DNA序列扩增后欧洲野牛κ-酪蛋白基因的基因分型
Genotyping of Bison bonasus kappa-casein gene following DNA sequence amplification.
作者信息
Burzynska B, Topczewski J
机构信息
Department of Genetics and Animal Breeding, Warsaw Agricultural University, Poland.
出版信息
Anim Genet. 1995 Oct;26(5):335-6. doi: 10.1111/j.1365-2052.1995.tb02669.x.
Abstract
DNA isolated from 30 Bison bonasus blood samples was used for amplification of a kappa-casein gene fragment to detect possible polymorphism. Bovine kappa-caseins exist in A, B and E variants. In the European bison from Poland, we have found only BB genotypes. Sequencing of the polymerase chain reaction product revealed further polymorphisms typical for B. bonasus.
摘要
从30份欧洲野牛血液样本中分离出的DNA用于扩增κ-酪蛋白基因片段，以检测可能的多态性。牛κ-酪蛋白存在A、B和E变体。在来自波兰的欧洲野牛中，我们仅发现了BB基因型。聚合酶链反应产物的测序揭示了欧洲野牛特有的更多多态性。
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