柳氏非染色氢氧化钾技术用于快速确定食源性和水源性细菌及酵母菌革兰氏反应的效果。
Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts.
作者信息
Powers E M
机构信息
U.S. Army Soldier System Command, Natick Research, Development and Engineering Center, Massachusetts 01760-5018, USA.
出版信息
Appl Environ Microbiol. 1995 Oct;61(10):3756-8. doi: 10.1128/aem.61.10.3756-3758.1995.
Abstract
A simple and rapid (< 60 s) nonstaining technique with 3% potassium hydroxide to determine Gram reactions was tested with 495 food-borne and waterborne bacteria and yeasts. In KOH, suspensions of gram-negative bacteria become viscous and string out. Gram-positive bacteria are not affected. There was 100% correlation between the KOH string test results and gram-positive and gram-negative strains.
摘要
采用一种简单快速（<60秒）的3%氢氧化钾非染色技术来确定革兰氏反应，对495株食源性和水源性细菌及酵母菌进行了测试。在氢氧化钾中，革兰氏阴性菌的悬液会变黏稠并拉丝。革兰氏阳性菌不受影响。氢氧化钾拉丝试验结果与革兰氏阳性和革兰氏阴性菌株之间存在100%的相关性。
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本文引用的文献
1
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