区分革兰氏阳性菌和革兰氏阴性菌的方法。
Methods for distinguishing gram-positive from gram-negative bacteria.
作者信息
Carlone G M, Valadez M J, Pickett M J
出版信息
J Clin Microbiol. 1982 Dec;16(6):1157-9. doi: 10.1128/jcm.16.6.1157-1159.1982.
Abstract
Lysis by KOH and hydrolysis of L-alanine-4-nitroanilide were compared with the Gram reaction of aerobic, microaerophilic, and anaerobic bacteria. Both tests correlated well with the Gram reaction with nonfermentative bacilli and Bacillus species, whereas they did not correlate with nonsporulating anaerobes. Only campylobacteria were KOH positive and L-alanine-4-nitroanilide and gram negative.
摘要
将氢氧化钾（KOH）裂解试验和L-丙氨酸-4-硝基苯胺水解试验与需氧菌、微需氧菌和厌氧菌的革兰氏反应进行了比较。这两种试验与非发酵杆菌和芽孢杆菌属的革兰氏反应相关性良好，而与无芽孢厌氧菌不相关。只有弯曲杆菌KOH试验呈阳性，L-丙氨酸-4-硝基苯胺试验呈阳性且革兰氏染色阴性。
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