Wickett R R, Nath V, Tanaka R, Hoath S B
College of Pharmacy, University of Cincinnaty, Ohio, USA.
Skin Pharmacol. 1995;8(4):179-85. doi: 10.1159/000211344.
A vital function of the skin is to oppose the loss of water to the environment. In this study, two complementary methods, transepidermal water loss (TEWL) and continuous electrical capacitance under occlusion, were used to assess epidermal barrier function in a developmental animal model, the neonatal Sprague-Dawley rat. TEWL monitors barrier function directly while the increase in capacitance under occlusion is related to both the skin's barrier function and to its water holding capacity. Serial tape stripping of the stratum corneum on 1-day-old rat pups led to a significant increase in both TEWL and continuous capacitance measurements. Anatomic site heterogeneity and the effects of surface temperature were also studied. The ventral skin surface exhibited an increase in the continuous capacitance measurements, an effect possibly due to the thinner stratum corneum on the ventral side. Both TEWL and continuous capacitance values were directly correlated with ambient temperatures within the physiological range.
皮肤的一项重要功能是防止水分流失到周围环境中。在本研究中，采用两种互补方法，即经表皮水分流失（TEWL）和封闭状态下的连续电容测量，来评估新生斯普拉格-道利大鼠这一发育动物模型的表皮屏障功能。TEWL直接监测屏障功能，而封闭状态下电容的增加与皮肤的屏障功能及其持水能力均相关。对1日龄幼鼠的角质层进行连续胶带剥离，导致TEWL和连续电容测量值均显著增加。还研究了解剖部位的异质性以及表面温度的影响。腹部皮肤表面的连续电容测量值有所增加，这一效应可能是由于腹侧角质层较薄所致。在生理范围内，TEWL和连续电容值均与环境温度直接相关。
