Lafrance H, Guillot M, Germain L, Auger F A
Laboratoire d'Organogénèse Expérimentale, Hôpital du Saint-Sacrement, Québec (Québec), Canada.
Med Eng Phys. 1995 Oct;17(7):537-43. doi: 10.1016/1350-4533(95)00012-c.
In vitro production of anchored skin equivalent is a new therapeutical option for burn patients. A skin equivalent is a combined culture of dermal and epidermal layers. The dermal layer provides important mechanical properties, such as tensile resistance and nonlinear elasticity, to the skin equivalent during its development. Prior to any in vivo human transplantation, the tensile properties of cutaneous equivalents have to be evaluated as a function of its structural components, in view of establishing the culture conditions leading to the best mechanical resistance and stretchability characteristics. However, the handling and clamping of skin equivalents are frequent causes of tearing and lack of repeatability in the measuring of tensile properties. A new indentation method involving a specially designed culture dish has been developed to minimize the risk of damage. Using this new culture dish, cutaneous equivalents were installed on an indentation apparatus. The central loading of a spherical tip was transmitted to the central area of a circular anchored cutaneous equivalent and was recorded with tip position. The tests were achieved at a constant low deflection rate of the tip. This new and accurate method gave repeatability in three central load-deflection characteristics of anchored dermal equivalent: the high-modulus (0.15 g mm-1), the central load of rupture (1.49 g), the rupture deflection (0.470 mm). This indentation test is expected to be an efficient tool in the evaluation of various skin equivalent models tensile properties.
体外制备锚定皮肤替代物是烧伤患者的一种新的治疗选择。皮肤替代物是真皮层和表皮层的联合培养物。在其发育过程中，真皮层为皮肤替代物提供重要的力学性能，如抗张性和非线性弹性。在进行任何体内人体移植之前，鉴于要确定能产生最佳机械抗性和拉伸特性的培养条件，必须根据其结构成分来评估皮肤替代物的拉伸性能。然而，在处理和夹持皮肤替代物时，经常会导致撕裂，并且在拉伸性能测量中缺乏可重复性。为了将损坏风险降至最低，已经开发出一种涉及特殊设计培养皿的新压痕方法。使用这种新的培养皿，将皮肤替代物安装在压痕装置上。球形尖端的中心载荷传递到圆形锚定皮肤替代物的中心区域，并随尖端位置进行记录。测试是在尖端恒定的低偏转速率下进行的。这种新的精确方法在锚定真皮替代物的三个中心载荷 - 偏转特性方面具有可重复性：高模量（0.15 g·mm -1）、破裂中心载荷（1.49 g）、破裂偏转（0.470 mm）。这种压痕测试有望成为评估各种皮肤替代物模型拉伸性能的有效工具。
