Ono K, Kawanaka Y, Izumi Y, Inui H, Miyatake K, Kitaoka S, Nakano Y
Department of Applied Biological Chemistry, University of Osaka Prefecture.
J Biochem. 1995 Jun;117(6):1178-82. doi: 10.1093/oxfordjournals.jbchem.a124841.
The inducing effects of ethanol on alcohol dehydrogenase and the key enzymes of the glyoxylate cycle, isocitrate lyase and malate synthase, in Euglena cells were investigated. Ethanol as the sole carbon source resulted in increases in alcohol dehydrogenase and the two glyoxylate cycle enzymes. The experimental results indicated that ethanol is assimilated by alcohol dehydrogenase and the glyoxylate cycle in Euglena. Mitochondria from aerobically grown Euglena contain a unique type of alcohol dehydrogenase that accounts for their ability to respire with ethanol as a substrate. This alcohol dehydrogenase was purified to homogeneity from ethanol-grown Euglena gracilis. The mitochondrial alcohol dehydrogenase was NAD(+)-specific but not NADP(+)-specific. Ethanol was the most active substrate, but the enzyme was also active towards 1-butanol, 1-heptanol, cinnamyl alcohol, and myristyl alcohol. These results indicated that mitochondrial alcohol dehydrogenase participated in alcohol metabolism in Euglena gracilis.
研究了乙醇对眼虫细胞中乙醇脱氢酶以及乙醛酸循环关键酶异柠檬酸裂解酶和苹果酸合酶的诱导作用。以乙醇作为唯一碳源会导致乙醇脱氢酶和两种乙醛酸循环酶的增加。实验结果表明，乙醇在眼虫中通过乙醇脱氢酶和乙醛酸循环被同化。需氧生长的眼虫的线粒体含有一种独特类型的乙醇脱氢酶，这解释了它们以乙醇作为底物进行呼吸的能力。从以乙醇培养的纤细眼虫中纯化该乙醇脱氢酶至同质。线粒体乙醇脱氢酶对NAD(+)具有特异性，而对NADP(+)不具有特异性。乙醇是最具活性的底物，但该酶对1-丁醇、1-庚醇、肉桂醇和肉豆蔻醇也有活性。这些结果表明线粒体乙醇脱氢酶参与了纤细眼虫中的酒精代谢。
