肺炎链球菌菌血症患者血清中C多糖的检测
Detection of C-polysaccharide in serum of patients with Streptococcus pneumoniae bacteraemia.
作者信息
Gillespie S H, Smith M D, Dickens A, Raynes J G, McAdam K P
机构信息
Department of Medical Microbiology, Royal Free Hospital, School of Medicine, London.
出版信息
J Clin Pathol. 1995 Sep;48(9):803-6. doi: 10.1136/jcp.48.9.803.
AIM
To investigate the fate of Streptococcus pneumoniae C-polysaccharide antigen in serum in patients with S pneumoniae bacteraemia.
METHOD
In vitro dissociation experiments were performed to demonstrate that C-polysaccharide was masked by ligands in normal and acute phase serum. Serum samples from 22 patients with S pneumoniae bacteraemia were treated to dissociate immune complexes and then tested for C-polysaccharide by enzyme linked immunosorbent assay (ELISA).
RESULTS
C-polysaccharide antigen was masked in normal and acute phase serum but could be released by EDTA treatment and detected by ELISA. Antigen was found in six patients ranging in concentration from 2.5 to 200 ng/ml. Patients with detectable antigen were more likely to die than those in whom antigen was not detected.
CONCLUSION
This study demonstrates that C-polysaccharide antigen commonly circulates in patients with S pneumoniae bacteraemia but its presence is masked by ligands present in serum.
目的
研究肺炎链球菌菌血症患者血清中肺炎链球菌C多糖抗原的转归。
方法
进行体外解离实验以证明在正常血清和急性期血清中C多糖被配体掩盖。对22例肺炎链球菌菌血症患者的血清样本进行处理以解离免疫复合物，然后通过酶联免疫吸附测定（ELISA）检测C多糖。
结果
C多糖抗原在正常血清和急性期血清中被掩盖，但可通过乙二胺四乙酸（EDTA）处理释放并通过ELISA检测到。在6例患者中发现了抗原，浓度范围为2.5至200 ng/ml。可检测到抗原的患者比未检测到抗原的患者更有可能死亡。
结论
本研究表明C多糖抗原在肺炎链球菌菌血症患者中普遍循环，但其存在被血清中的配体掩盖。
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