通过C多糖抗原的定量酶联免疫吸附测定诊断肺炎链球菌肺炎
Diagnosis of Streptococcus pneumoniae pneumonia by quantitative enzyme linked immunosorbent assay of C-polysaccharide antigen.
作者信息
Gillespie S H, Smith M D, Dickens A, Raynes J G, McAdam K P
机构信息
Division of Communicable Diseases, Royal Free Hospital School of Medicine, London.
出版信息
J Clin Pathol. 1994 Aug;47(8):749-51. doi: 10.1136/jcp.47.8.749.
AIMS
To evaluate the use of a quantitative enzyme linked immunosorbent assay (ELISA) detecting C-polysaccharide (PnC) antigen in sputum for the diagnosis of Streptococcus pneumoniae infection.
METHODS
Specimens of sputum from 60 patients with acute community and hospital acquired pneumonia and infective exacerbations of obstructive airways disease were examined by semiquantitative culture and antigen ELISA.
RESULTS
Using a cutoff value of 1 microgram/ml PnC antigen for a positive result, the sensitivity of this assay was 90.3%, specificity 93.1%, predictive value of a positive result was 93.5%, and the predictive value of a negative result 89.6%.
CONCLUSIONS
Quantitation of C-polysaccharide antigen in sputum by ELISA distinguishes between carriage of oral bacteria which express PnC-like antigen and infection with S pneumoniae and compares favourably with other diagnostic methods.
目的
评估使用定量酶联免疫吸附测定（ELISA）检测痰液中C多糖（PnC）抗原以诊断肺炎链球菌感染的情况。
方法
对60例急性社区获得性和医院获得性肺炎以及阻塞性气道疾病感染加重患者的痰液标本进行半定量培养和抗原ELISA检测。
结果
以1微克/毫升PnC抗原作为阳性结果的临界值，该检测方法的敏感性为90.3%，特异性为93.1%，阳性预测值为93.5%，阴性预测值为89.6%。
结论
通过ELISA对痰液中的C多糖抗原进行定量分析，可区分表达PnC样抗原的口腔细菌定植与肺炎链球菌感染，与其他诊断方法相比具有优势。
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