Adegboye D S
Res Vet Sci. 1978 Nov;25(3):323-30.
Measurements of lymphocyte transformation and skin sensitivity were used to investigate whether pigs previously inoculated with Mycoplasma suipneumoniae would show a T-lymphocyte-mediated immune response. In the lymphocyte transformation test blood lymphocytes, obtained at various intervals after intranasal inoculation of M suipneumoniae cultures, were incubated with specific antigen and the degree of transformation was assessed by 14C-thymidine uptake. Reaction to intradermal antigen was assessed both grossly and histologically. Significant lymphocyte transformation was first obtained at approximately 15 weeks and was demonstrable up to the maximum observation period of 44 weeks after inoculation; this corresponded to the stage of progressive recovery from infection earlier described. Skin hypersensitivity, characterised histologically by perivascular mononuclear cell accumulation in the dermis, was demonstrated in all tests performed between 20 and 46 weeks after inoculation on previously infected pigs, and was apparently enhanced by repeated intranasal inoculation. Both types of responses were more marked in pigs inoculated with pathogenic M suipneumoniae than in pigs inoculated with attenuated M suipneumoniae.
采用淋巴细胞转化试验和皮肤敏感性检测，研究先前接种过猪肺炎支原体的猪是否会出现T淋巴细胞介导的免疫反应。在淋巴细胞转化试验中，于鼻内接种猪肺炎支原体培养物后的不同时间点采集血液淋巴细胞，与特异性抗原一起孵育，并通过¹⁴C - 胸腺嘧啶核苷摄取来评估转化程度。通过大体观察和组织学方法评估对皮内抗原的反应。接种后约15周首次观察到显著的淋巴细胞转化，并且在接种后长达44周的最长观察期内均可检测到；这与先前描述的感染后逐渐恢复阶段相对应。皮肤超敏反应在接种后20至46周对先前感染的猪进行的所有试验中均有表现，其组织学特征为真皮血管周围单核细胞积聚，并且通过重复鼻内接种明显增强。接种致病性猪肺炎支原体的猪的这两种反应比接种减毒猪肺炎支原体的猪更为明显。
