Levi M I, Livshits M M, Apanovskiĭ L N
Zh Mikrobiol Epidemiol Immunobiol. 1977 Dec(12):101-8.
By the serological tests with the erythrocytic diagnostic agents it is possible to estimate the concentration of the determinants in the antigenic preparation under study. For this purpose it is necessary to have at one's disposal the data on the molecular weight and concentration of the reference antigen, valence of erythrocytes loaded with the antigen or antibodies, and antibody heterogeneity index. If these values are known, then one can calculate the concentration of antigenic determinants in terms divided by the valence of the reference antigen. With the aid of pure antibodies preparation obtained with the immunosorption procedure it is possible to measure the determinant concentration of the antigen tested and the valence of the reference antigen. For the capsular antigen of plaque bacilli the valence was estimated by two independent methods and in both cases it was close to 10. The valence of the reference capsular antigen permits to calculate the concentration of the antibody molecules in the hyperimmune sera without isolation of pure antibodies.
通过使用红细胞诊断剂进行血清学检测，可以估计所研究抗原制剂中决定簇的浓度。为此，必须掌握有关参考抗原的分子量和浓度、负载抗原或抗体的红细胞效价以及抗体异质性指数的数据。如果知道这些值，那么就可以以参考抗原效价为除数来计算抗原决定簇的浓度。借助免疫吸附法获得的纯抗体制剂，可以测量被测抗原的决定簇浓度和参考抗原的效价。对于鼠疫杆菌的荚膜抗原，通过两种独立方法估计其效价，在两种情况下其效价均接近10。参考荚膜抗原的效价允许在不分离纯抗体的情况下计算超免疫血清中抗体分子的浓度。
