Levi M I
Zh Mikrobiol Epidemiol Immunobiol. 1976 Aug(8):103-10.
Agglutination of erythrocytes of the antigenic diagnostic agents with the IgG antibodies is attributed to the fact that the reactive centres of the same molecule of the antibody attached to the antigenic determinants located on different erythrocytes. A possibility of agglutination of erythrocytes under conditions of the maximal dilution of the immune serum depended on the number of molecules located on a single erythrocyte. When the erythrocytes are sensitized with the optimal dose of the antigen those serum antibodies are involved in the passive hemagglutination test (PHAT) which are characterized by the mean integrated association constant. When the antibodies participate in the PHAT with several batches of the erythrocytic diagnostic agent characterized by an average number of the antigen molecules on a single erythrocyte there appears a possibility of determining the extent of heterogeneity of the antibodies. Not only the amount of antibodies, but also their affinity can be ascertained by the PHAT, and also the heterogeneity of antibodies can be evaluated.
抗原诊断剂的红细胞与IgG抗体的凝集归因于这样一个事实，即附着在位于不同红细胞上的抗原决定簇上的抗体同一分子的反应中心。在免疫血清最大稀释条件下红细胞发生凝集的可能性取决于单个红细胞上的分子数量。当红细胞用最佳剂量的抗原致敏时，那些具有平均综合结合常数特征的血清抗体参与被动血凝试验（PHAT）。当抗体与几批以单个红细胞上抗原分子平均数为特征的红细胞诊断剂一起参与PHAT时，就有可能确定抗体的异质性程度。通过PHAT不仅可以确定抗体的数量，还可以确定其亲和力，并且还可以评估抗体的异质性。
