Masalski N
Vet Med Nauki. 1978;15(7):31-7.
Two killed vaccines were produced, differing by the adjuvant used--a hydroxide one and an oil one--employing four strains of Brucella ovis. They were applied twice at an interval of 20 days to rams under experimental conditions as well as to rams in filed conditions. On the 45th day following vaccination the experimental rams were challenged with a virulent Brucella ovis strain. To determine the acquired resistance the rams were investigated after the complement-fixation test, hemagglutination reaction, and agar gel immunodiffusion as well as through clinical and bacteriological examination of sampled semen for the presence of bacteria. It was established that rams treated with an oil vaccine built up a solid immunity. The postvaccinal antibodies disappeared after the third month of vaccination. It is suggested to carry out vaccinations with rams of the infected flocks each 6 months. The infected animals can be identified by means of serologic examination three months following vaccination. Vaccination makes it possible to use valuable animals for a longer period, which, though infected are not hazardous in spreading the infection in the flock. A programme is referred to for the curing of flocks by means of vaccination applied to the intact rams and gradual elimination of the infected animals.
生产了两种灭活疫苗，它们的区别在于所使用的佐剂——一种是氢氧化铝佐剂，另一种是油佐剂——使用了四株绵羊布鲁氏菌。在实验条件下以及在实际养殖条件下，每隔20天给公羊接种两次。接种后第45天，用强毒绵羊布鲁氏菌菌株对实验公羊进行攻毒。为了确定获得性抵抗力，对公羊进行了补体结合试验、血凝反应、琼脂凝胶免疫扩散检测，并对采集的精液进行临床和细菌学检查以检测细菌的存在。结果表明，用油佐剂疫苗处理的公羊产生了牢固的免疫力。接种后抗体在第三个月后消失。建议每隔6个月对感染羊群的公羊进行一次接种。接种三个月后可通过血清学检查识别感染动物。接种疫苗使得可以更长时间地利用有价值的动物，这些动物虽然感染了，但在羊群中传播感染并无危害。文中提到了一个通过对未感染的公羊接种疫苗并逐步淘汰感染动物来治愈羊群的方案。
