结核分枝杆菌对吡嗪酰胺的药敏试验：Bactec法与吡嗪酰胺酶测定法的比较
Testing of susceptibility of Mycobacterium tuberculosis to pyrazinamide: comparison of Bactec method with pyrazinamidase assay.
作者信息
Miller M A, Thibert L, Desjardins F, Siddiqi S H, Dascal A
机构信息
McGill University, Montreal, Quebec, Canada.
出版信息
J Clin Microbiol. 1995 Sep;33(9):2468-70. doi: 10.1128/jcm.33.9.2468-2470.1995.
Abstract
The susceptibility of 428 clinical isolates of Mycobacterium tuberculosis to pyrazinamide was assessed by the Bactec method and the Wayne pyrazinamidase assay. The correlation between the two tests was 98.2 and 100% for susceptible and resistant strains, respectively. False resistance was seen in four (0.8%) strains with the Bactec test, and false-susceptible results occurred in two (0.5%) pyrazinamidase assays. The Bactec test is rapid and reliable, and the Bactec results correlate well with the pyrazinamidase test results, although some strains did not grow well in the test medium.
摘要
采用Bactec法和韦恩吡嗪酰胺酶测定法评估了428株结核分枝杆菌临床分离株对吡嗪酰胺的敏感性。对于敏感菌株和耐药菌株，两种检测方法的相关性分别为98.2%和100%。在Bactec检测中有4株（0.8%）菌株出现假耐药，在吡嗪酰胺酶测定中有2株（0.5%）出现假敏感结果。Bactec检测快速可靠，尽管有些菌株在测试培养基中生长不佳，但Bactec检测结果与吡嗪酰胺酶检测结果相关性良好。
相似文献
1
Testing of susceptibility of Mycobacterium tuberculosis to pyrazinamide: comparison of Bactec method with pyrazinamidase assay.结核分枝杆菌对吡嗪酰胺的药敏试验：Bactec法与吡嗪酰胺酶测定法的比较
J Clin Microbiol. 1995 Sep;33(9):2468-70. doi: 10.1128/jcm.33.9.2468-2470.1995.
2
Potential for erroneous results indicating resistance when using the Bactec MGIT 960 system for testing susceptibility of Mycobacterium tuberculosis to pyrazinamide.使用 Bactec MGIT 960 系统检测结核分枝杆菌对吡嗪酰胺的敏感性时，可能会出现错误的耐药结果。
J Clin Microbiol. 2010 Jan;48(1):300-1. doi: 10.1128/JCM.01775-09. Epub 2009 Nov 18.
3
Evaluating current methods for determination of the susceptibility of mycobacteria to pyrazinamide, conventional, radiometric Bactec and two methods of pyrazinamidase testing.
Lett Appl Microbiol. 1997 Feb;24(2):127-32. doi: 10.1046/j.1472-765x.1997.00367.x.
4
Comparison of phenotypic and genotypic methods for pyrazinamide susceptibility testing with Mycobacterium tuberculosis.结核分枝杆菌吡嗪酰胺药敏试验的表型和基因型方法比较
J Clin Microbiol. 2000 Oct;38(10):3686-8. doi: 10.1128/JCM.38.10.3686-3688.2000.
5
Pyrazinoic acid efflux rate in Mycobacterium tuberculosis is a better proxy of pyrazinamide resistance.吡嗪酸外排率是结核分枝杆菌吡嗪酰胺耐药的更好替代指标。
Tuberculosis (Edinb). 2012 Jan;92(1):84-91. doi: 10.1016/j.tube.2011.09.002. Epub 2011 Oct 17.
6
Evaluation of the BacT/ALERT PZA kit in comparison with the BACTEC 460TB PZA for testing Mycobacterium tuberculosis susceptibility to pyrazinamide.BacT/ALERT PZA试剂盒与BACTEC 460TB PZA用于检测结核分枝杆菌对吡嗪酰胺敏感性的比较评估。
J Antimicrob Chemother. 2007 Sep;60(3):655-7. doi: 10.1093/jac/dkm252. Epub 2007 Jul 5.
7
A simplified pyrazinamidase test for pyrazinamide drug susceptibility in Mycobacterium tuberculosis.一种用于检测结核分枝杆菌对吡嗪酰胺药物敏感性的简化吡嗪酰胺酶试验。
J Microbiol Methods. 2018 Nov;154:52-54. doi: 10.1016/j.mimet.2018.09.018. Epub 2018 Oct 11.
8
A new rapid and simple colorimetric method to detect pyrazinamide resistance in Mycobacterium tuberculosis using nicotinamide.一种使用烟酰胺检测结核分枝杆菌对吡嗪酰胺耐药性的快速简便比色新方法。
J Antimicrob Chemother. 2006 Aug;58(2):327-31. doi: 10.1093/jac/dkl231. Epub 2006 Jun 2.
9
[Evaluation of the pyrazinamidase test for the detection of susceptibility of Mycobacterium tuberculosis to pyrazinamide].[用于检测结核分枝杆菌对吡嗪酰胺敏感性的吡嗪酰胺酶试验的评估]
Rev Cubana Med Trop. 1999 Jan-Apr;51(1):53-4.
引用本文的文献
1
Prediction of pyrazinamide resistance in using structure-based machine-learning approaches.使用基于结构的机器学习方法预测吡嗪酰胺耐药性。 （你提供的原文“in using”表述有误，可能是“by using”之类的，我按照正确理解翻译了。）
JAC Antimicrob Resist. 2024 Mar 18;6(2):dlae037. doi: 10.1093/jacamr/dlae037. eCollection 2024 Apr.
2
Structure guided prediction of Pyrazinamide resistance mutations in pncA.基于结构的结核分枝杆菌 pncA 中吡嗪酰胺耐药突变预测。
Sci Rep. 2020 Feb 5;10(1):1875. doi: 10.1038/s41598-020-58635-x.
3
Design, Synthesis and Antitubercular Evaluation of Novel Series of Pyrazinecarboxamide Metal Complexes.新型吡嗪甲酰胺金属配合物系列的设计、合成与抗结核活性评价
Iran J Pharm Res. 2018 Winter;17(1):93-99.
4
A comprehensive characterization of PncA polymorphisms that confer resistance to pyrazinamide.对赋予吡嗪酰胺抗性的PncA多态性的全面表征。
Nat Commun. 2017 Sep 19;8(1):588. doi: 10.1038/s41467-017-00721-2.
5
Systematic review of mutations in pyrazinamidase associated with pyrazinamide resistance in Mycobacterium tuberculosis clinical isolates.结核分枝杆菌临床分离株中与吡嗪酰胺耐药相关的吡嗪酰胺酶突变的系统评价
Antimicrob Agents Chemother. 2015 Sep;59(9):5267-77. doi: 10.1128/AAC.00204-15. Epub 2015 Jun 15.
6
'Z(S)-MDR-TB' versus 'Z(R)-MDR-TB': improving treatment of MDR-TB by identifying pyrazinamide susceptibility.“Z(S)-耐多药结核病”与“Z(R)-耐多药结核病”：通过确定吡嗪酰胺敏感性改善耐多药结核病的治疗
Emerg Microbes Infect. 2012 Jul;1(7):e5. doi: 10.1038/emi.2012.18. Epub 2012 Jul 25.
7
Rapid detection of Mycobacterium tuberculosis and pyrazinamide susceptibility related to pncA mutations in sputum specimens through an integrated gene-to-protein function approach.通过整合基因到蛋白质功能的方法，快速检测痰标本中与 pncA 突变相关的结核分枝杆菌和吡嗪酰胺药敏性。
J Clin Microbiol. 2014 Jan;52(1):260-7. doi: 10.1128/JCM.02285-13. Epub 2013 Nov 13.
8
Evaluation of methods for testing the susceptibility of clinical Mycobacterium tuberculosis isolates to pyrazinamide.临床分枝杆菌分离株对吡嗪酰胺敏感性检测方法的评价。
J Clin Microbiol. 2013 May;51(5):1374-80. doi: 10.1128/JCM.03197-12. Epub 2013 Feb 6.
9
Prevention of false resistance results obtained in testing the susceptibility of Mycobacterium tuberculosis to pyrazinamide with the Bactec MGIT 960 system using a reduced inoculum.使用减菌接种量预防 Bactec MGIT 960 系统检测结核分枝杆菌对吡嗪酰胺敏感性时出现假耐药结果。
J Clin Microbiol. 2013 Jan;51(1):291-4. doi: 10.1128/JCM.01838-12. Epub 2012 Oct 24.
10
Role of metal ions on the activity of Mycobacterium tuberculosis pyrazinamidase.金属离子对结核分枝杆菌吡嗪酰胺酶活性的影响。
Am J Trop Med Hyg. 2012 Jul;87(1):153-61. doi: 10.4269/ajtmh.2012.10-0565.
本文引用的文献
1
Comparison of growth and susceptibility testing of pyrazinamide in different Bactec media using strains of the M. tuberculosis complex.
APMIS. 1993 Feb;101(2):154-9. doi: 10.1111/j.1699-0463.1993.tb00095.x.
2
Improved method for testing susceptibility of Mycobacterium tuberculosis to pyrazinamide.检测结核分枝杆菌对吡嗪酰胺敏感性的改良方法。
J Clin Microbiol. 1982 Dec;16(6):1106-9. doi: 10.1128/jcm.16.6.1106-1109.1982.
3
Use of pyrazinamidase activity on Mycobacterium tuberculosis as a rapid method for determination of pyrazinamide susceptibility.利用结核分枝杆菌中的吡嗪酰胺酶活性作为快速测定吡嗪酰胺敏感性的方法。
Antimicrob Agents Chemother. 1981 Oct;20(4):556-7. doi: 10.1128/AAC.20.4.556.
4
Susceptibility of Mycobacterium tuberculosis to pyrazinamide and its relationship to pyrazinamidase activity.结核分枝杆菌对吡嗪酰胺的敏感性及其与吡嗪酰胺酶活性的关系。
Antimicrob Agents Chemother. 1983 Oct;24(4):600-1. doi: 10.1128/AAC.24.4.600.
5
Probability distribution of drug-resistant mutants in unselected populations of Mycobacterium tuberculosis.结核分枝杆菌未选择群体中耐药突变体的概率分布。
Appl Microbiol. 1970 Nov;20(5):810-4. doi: 10.1128/am.20.5.810-814.1970.
6
Determination of drug susceptibility of mycobacteria to pyrazinamide in 7H10 agar.
Am Rev Respir Dis. 1967 Nov;96(5):1072-5. doi: 10.1164/arrd.1967.96.5.1072.
7
Simple pyrazinamidase and urease tests for routine identification of mycobacteria.用于分枝杆菌常规鉴定的简易吡嗪酰胺酶和脲酶试验
Am Rev Respir Dis. 1974 Jan;109(1):147-51. doi: 10.1164/arrd.1974.109.1.147.
8
Determination of pyrazinamide MICs for Mycobacterium tuberculosis at different pHs by the radiometric method.采用放射测量法测定不同pH值下结核分枝杆菌对吡嗪酰胺的最低抑菌浓度。
Antimicrob Agents Chemother. 1988 Jul;32(7):1002-4. doi: 10.1128/AAC.32.7.1002.
9
Pyrazinamidase activity of Mycobacterium tuberculosis--a test of sensitivity to pyrazinamide.
Tubercle. 1987 Sep;68(3):221-4. doi: 10.1016/0041-3879(87)90058-4.
10
Susceptibility testing of Mycobacterium tuberculosis to pyrazinamide.结核分枝杆菌对吡嗪酰胺的药敏试验。
Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Jul;265(3-4):404-7.
Zotero 插件