Suresh M, Karaca K, Foster D, Sharma J M
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
J Virol. 1995 Dec;69(12):8159-63. doi: 10.1128/JVI.69.12.8159-8163.1995.
The turkey interferon (TkIFN) gene encodes a signal peptide and a mature protein of 30 and 162 amino acids, respectively. TkIFN mRNA expression was induced by reoviral double-stranded RNA in fibroblasts. The recombinant TkIFN protein possessed species-specific antiviral activity and in synergy with lipopolysaccharide (LPS) induced bone marrow macrophages to produce nitric oxide (NO). LPS or TkIFN alone did not induce bone marrow macrophages to produce significant amounts of NO, which showed that TkIFN provided one of the two signals necessary to induce NO production in turkey macrophages. Unlike the anti-inflammatory nature of mammalian alpha/beta IFNs, TkIFN augmented the LPS-induced expression of interleukin-8, a proinflammatory cytokine. This finding suggests a role for TkIFN in inflammatory conditions.
火鸡干扰素（TkIFN）基因分别编码一个由30个氨基酸组成的信号肽和一个由162个氨基酸组成的成熟蛋白。呼肠孤病毒双链RNA可诱导成纤维细胞中TkIFN mRNA的表达。重组TkIFN蛋白具有种属特异性抗病毒活性，并与脂多糖（LPS）协同作用诱导骨髓巨噬细胞产生一氧化氮（NO）。单独的LPS或TkIFN均不能诱导骨髓巨噬细胞产生大量的NO，这表明TkIFN是诱导火鸡巨噬细胞产生NO所需的两个信号之一。与哺乳动物α/β干扰素的抗炎性质不同，TkIFN增强了LPS诱导的促炎细胞因子白细胞介素-8的表达。这一发现提示TkIFN在炎症状态中发挥作用。
