念珠菌抗原滴度升高与损伤后中性粒细胞功能障碍有关。
Elevated Candida antigen titers are associated with neutrophil dysfunction after injury.
作者信息
Sweeney J F, Rosemurgy A S, Wei S, Djeu J Y
机构信息
Department of Surgery, University of South Florida College of Medicine, Tampa 33612, USA.
出版信息
Clin Diagn Lab Immunol. 1994 Jan;1(1):111-4. doi: 10.1128/cdli.1.1.111-114.1994.
This study was undertaken to determine if impaired neutrophil (polymorphonuclear leukocytes [PMNL]) function is associated with an elevated Candida antigen titer after injury. PMNL from eight severely injured adults with Candida antigen titers of > or = 1:4 (titer positive) were evaluated for the ability to inhibit growth of Candida albicans in vitro by using a [3H]glucose incorporation assay. PMNL from eight severely injured adults with titers of < 1:4 (titer negative) and from eight healthy volunteers were studied for comparison. PMNL from the titer-positive patients had suppressed ability to inhibit C. albicans growth compared with PMNL from titer-negative patients and healthy volunteers. In vitro, PMNL function against C. albicans could be augmented significantly by cytokines. Granulocyte macrophage-colony-stimulating factor was most potent at augmenting function, followed by interleukin-8 and gamma interferon. Injured patients with elevated candida antigen titers have impaired PMNL function against C. albicans, and this function can be restored by cytokines.
本研究旨在确定中性粒细胞（多形核白细胞[PMNL]）功能受损是否与损伤后念珠菌抗原滴度升高有关。对8名念珠菌抗原滴度≥1:4（滴度阳性）的严重受伤成年人的PMNL，采用[3H]葡萄糖掺入试验评估其体外抑制白色念珠菌生长的能力。对8名滴度<1:4（滴度阴性）的严重受伤成年人及8名健康志愿者的PMNL进行研究以作比较。与滴度阴性患者和健康志愿者的PMNL相比，滴度阳性患者的PMNL抑制白色念珠菌生长的能力受到抑制。在体外，细胞因子可显著增强PMNL针对白色念珠菌的功能。粒细胞巨噬细胞集落刺激因子增强功能的作用最强，其次是白细胞介素-8和γ干扰素。念珠菌抗原滴度升高的受伤患者的PMNL针对白色念珠菌的功能受损，而这种功能可通过细胞因子恢复。
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