Arias C F, López S, Mascarenhas J D, Romero P, Cano P, Gabbay Y B, de Freitas R B, Linhares A C
Departamento de Biología Molecular, Universidad Nacional Autónoma de México, Cuernavaca, Mexico.
Clin Diagn Lab Immunol. 1994 Jan;1(1):89-94. doi: 10.1128/cdli.1.1.89-94.1994.
We have characterized the neutralizing antibody immune response to six human rotavirus serotypes (G1 to G4, G8, and G9) in Brazilian children with primary and secondary rotavirus infections and correlated the response with the G serotype of the infecting rotavirus strain. Twenty-five children were studied: 17 had a single rotavirus infection, 4 were reinfected once, and 4 experienced three infections. Two of the reinfections were by non-group A rotaviruses. Among the 25 primary infections, we observed homotypic as well as heterotypic responses; the serotype G1 viruses, which accounted for 13 of these infections, induced mostly a homotypic response, while infections by serotype G2 and G4 viruses induced, in addition to the homotypic, a heterotypic response directed primarily to serotype G1. Two of the primary infections induced heterotypic antibodies to 69M, a serotype G8 virus that by RNA electrophoresis analysis was found not to circulate in the population during the time of the study. The specificity of the neutralizing antibody immune response induced by a virus of a given serotype was the same in primary as well as secondary infections. These results indicate that the heterotypic immune response induced in a primary rotavirus infection is an intrinsic property of the virus strain, and although there seem to be general patterns of serotype-specific seroconversion, these may vary from serotype to serotype and from strain to strain within a serotype.
我们已对巴西原发性和继发性轮状病毒感染儿童针对六种人类轮状病毒血清型（G1至G4、G8和G9）的中和抗体免疫反应进行了特征分析，并将该反应与感染性轮状病毒株的G血清型相关联。对25名儿童进行了研究：17名儿童有单次轮状病毒感染，4名儿童再次感染一次，4名儿童经历了三次感染。其中两次再次感染是由非A组轮状病毒引起的。在这25次原发性感染中，我们观察到了同型以及异型反应；血清型G1病毒导致了其中13次感染，大多诱导了同型反应，而血清型G2和G4病毒感染除了诱导同型反应外，还诱导了主要针对血清型G1的异型反应。两次原发性感染诱导了针对69M的异型抗体，69M是一种血清型G8病毒，通过RNA电泳分析发现该病毒在研究期间未在人群中传播。给定血清型病毒诱导的中和抗体免疫反应的特异性在原发性和继发性感染中是相同的。这些结果表明，原发性轮状病毒感染中诱导的异型免疫反应是病毒株的固有特性，尽管似乎存在血清型特异性血清转化的一般模式，但这些模式可能因血清型不同以及同一血清型内的毒株不同而有所差异。
