Offit P A, Hoffenberg E J, Santos N, Gouvea V
Division of Allergy, Immunology and Infectious Diseases, Children's Hospital of Philadelphia, PA 19104.
J Infect Dis. 1993 Jun;167(6):1436-40. doi: 10.1093/infdis/167.6.1436.
The humoral and cellular immune response after symptomatic, primary rotavirus infection was examined in 8 children < 2 years old. Rotavirus-specific IgA, rotavirus-specific helper T (Th) cells, and neutralizing antibody responses were evaluated at the time of illness, 2-8 weeks later, and 3-5 months later. In addition, rotavirus strains associated with infection were tested by polymerase chain reaction analysis using oligonucleotide primers specific for genes 4 (P type) and 9 (G type). The absence of rotavirus-specific IgA or rotavirus-specific helper T cell activity at the time of illness was consistent with a primary infection in 7 of 8 children. Two children were infected with serotype 1 (P type 1, G type 1), 3 with serotype 3 (P type 1, G type 3), and 3 with serotype 4 (P type 1, G type 4) strains. Neutralizing antibodies were directed against the gene 4 (P type) protein product (vp4). Because all infecting strains were P type 1, convalescent antisera did not distinguish among different rotavirus G types. Rotavirus-specific IgA responses were detected in 6 of 8 and rotavirus-specific Th cell responses in 7 of 8 children during convalescence.
对8名2岁以下出现症状的原发性轮状病毒感染儿童的体液免疫和细胞免疫反应进行了检测。在发病时、2 - 8周后以及3 - 5个月后评估了轮状病毒特异性IgA、轮状病毒特异性辅助性T（Th）细胞和中和抗体反应。此外，使用针对基因4（P型）和9（G型）的寡核苷酸引物，通过聚合酶链反应分析对与感染相关的轮状病毒株进行了检测。8名儿童中有7名在发病时缺乏轮状病毒特异性IgA或轮状病毒特异性辅助性T细胞活性，这与原发性感染一致。两名儿童感染了1型血清型（P型1，G型1），3名感染了3型血清型（P型1，G型3），3名感染了4型血清型（P型1，G型4）毒株。中和抗体针对基因4（P型）蛋白产物（vp4）。由于所有感染毒株均为P型1，恢复期抗血清无法区分不同的轮状病毒G型。8名儿童中有6名在恢复期检测到轮状病毒特异性IgA反应，8名中有7名检测到轮状病毒特异性Th细胞反应。
