Carl M, Isaacs S N, Kaur M, He J, Tam A W, Yarbough P O, Reyes G R
Accelerated Product Development Program, Naval Medical Research Institute, Bethesda, Maryland, USA.
Clin Diagn Lab Immunol. 1994 Mar;1(2):253-6. doi: 10.1128/cdli.1.2.253-256.1994.
Hepatitis E virus (HEV) is a polyadenylated, positive-stranded RNA virus which is a major cause of enterically transmitted non-A, non-B hepatitis in many developing countries. The viral genome contains three different open reading frames (ORFs): ORF1, which is believed to encode nonstructural proteins, and ORF2 and ORF3, which are believed to encode structural proteins. The full-length putative structural proteins encoded by ORF2 and ORF3 of HEV have been cloned and expressed in recombinant vaccinia virus. Proteins encoded by ORF2 and ORF3 when expressed in vaccinia virus are recognized by pooled sera obtained from individuals with acute hepatitis E. Vaccinia-expressed viral gene products of HEV will have utility in characterizing the cell-mediated immune response to HEV.
戊型肝炎病毒（HEV）是一种多聚腺苷酸化的正链RNA病毒，在许多发展中国家，它是经肠道传播的非甲非乙型肝炎的主要病因。病毒基因组包含三个不同的开放阅读框（ORF）：ORF1，被认为编码非结构蛋白；以及ORF2和ORF3，被认为编码结构蛋白。戊型肝炎病毒ORF2和ORF3编码的全长推定结构蛋白已在重组痘苗病毒中克隆并表达。当在痘苗病毒中表达时，ORF2和ORF3编码的蛋白可被急性戊型肝炎患者的混合血清识别。戊型肝炎病毒痘苗表达的病毒基因产物将有助于表征针对戊型肝炎病毒的细胞介导免疫反应。
