Wemmer T, Klämbt C
Institut für Entwicklungsbiologie, Universität zu Köln, Germany.
Genetics. 1995 Jun;140(2):629-41. doi: 10.1093/genetics/140.2.629.
The Drosophila gene argos encodes a diffusible protein that acts as a negative regulator of cell fate decisions. To define interacting gene products, we performed a genetic analysis of argos, which suggests the presence of several partially redundant gene functions in its immediate vicinity at the chromosomal position 73A. Dose titration experiments have identified two of these loci. One of them corresponds to the gene bulge. Loss of function bulge alleles suppress the rough eye phenotype associated with overexpression of argos; conversely, amorphic argos mutations suppress the eye phenotype seen in flies bearing a single dominant bulge allele. Recombination mapping localized bulge 0.15 cM distal to argos. A second gene, suppressor of bulge and argos (soba), corresponds to the recently described lethal complementation group 73Aj. soba alleles suppress the eye phenotypes seen in flies expressing either the dominant bulge allele or the hs-argos construct. soba resides 120 kb proximal to argos. In addition, we have identified one allele of a new gene, clown, which like soba suppresses the eye phenotypes associated with hs-argos and bulgeDominant. clown maps on chromosome 3 at the cytological position 68CD.
果蝇基因argos编码一种可扩散的蛋白质，它作为细胞命运决定的负调节因子。为了确定相互作用的基因产物，我们对argos进行了遗传分析，这表明在其染色体位置73A的紧邻区域存在几种部分冗余的基因功能。剂量滴定实验已经确定了其中两个位点。其中一个对应于基因bulge。功能缺失的bulge等位基因抑制了与argos过表达相关的粗糙眼表型；相反，无义argos突变抑制了携带单个显性bulge等位基因的果蝇中出现的眼表型。重组图谱将bulge定位在argos远端0.15 cM处。第二个基因，bulge和argos的抑制因子（soba），对应于最近描述的致死互补群73Aj。soba等位基因抑制了在表达显性bulge等位基因或hs - argos构建体的果蝇中出现的眼表型。soba位于argos近端120 kb处。此外，我们还鉴定了一个新基因clown的一个等位基因，它与soba一样，抑制了与hs - argos和bulgeDominant相关的眼表型。clown定位于3号染色体上细胞学位置68CD处。
