在果蝇中鉴定出两种I型骨形态发生蛋白受体，并证明Brk25D是一种果蝇dpp受体。

Identification of two bone morphogenetic protein type I receptors in Drosophila and evidence that Brk25D is a decapentaplegic receptor.

作者信息

Penton A, Chen Y, Staehling-Hampton K, Wrana J L, Attisano L, Szidonya J, Cassill J A, Massagué J, Hoffmann F M

机构信息

McArdle Laboratory for Cancer Research, Medical School, University of Wisconsin, Madison 53706.

出版信息

Cell. 1994 Jul 29;78(2):239-50. doi: 10.1016/0092-8674(94)90294-1.

DOI:10.1016/0092-8674(94)90294-1

PMID:8044838

Abstract

Drosophila sequences at chromosomal positions 25D (Brk25D) and 43E (Brk43E) are similar to the TGF beta type I receptor serine/threonine kinases and are expressed broadly during embryogenesis. Brk25D binds dpp protein and bone morphogenetic protein 2 with high affinity. Mutations affecting Brk25D map to the gene thick veins and block the expression of two decapentaplegic-responsive (dpp-responsive) genes, dpp and labial, in the embryonic midgut. Defects in Brk25D receptor function combined with reduced expression of dpp ligand produce mutant phenotypes in the embryo and adult. Brk43E is the product of the gene saxophone, which also interacts with dpp. We conclude that dpp signaling in vivo is mediated by at least two receptors, Brk25D and Brk43E.

摘要

位于染色体位置25D（Brk25D）和43E（Brk43E）的果蝇序列与转化生长因子β I型受体丝氨酸/苏氨酸激酶相似，并且在胚胎发生过程中广泛表达。Brk25D以高亲和力结合dpp蛋白和骨形态发生蛋白2。影响Brk25D的突变定位到厚脉基因，并阻断胚胎中肠中两个对dpp有反应（dpp反应性）的基因——dpp和唇的表达。Brk25D受体功能缺陷与dpp配体表达降低相结合，在胚胎和成虫中产生突变表型。Brk43E是萨克斯管基因的产物，它也与dpp相互作用。我们得出结论，体内dpp信号传导至少由两种受体Brk25D和Brk43E介导。