Takatsuki F, Namiki R, Kikuchi T, Suzuki M, Hamuro J
Basic Research Laboratories Ajinomoto Co., Inc., Kawasaki, Japan.
Int J Immunopharmacol. 1995 Jun;17(6):465-74. doi: 10.1016/0192-0561(95)00037-3.
The effects of lentinan, an antitumor polysaccharide, on vascular reactions against vasoactive mediators were investigated in murine systems. Lentinan augmented intradermal reactions against bradykinin. Induction of acute phase proteins (APP) and the vascular dilatation hemorrhage (VDH) reaction on the ears have been reported to reflect the host responses to lentinan. The strain difference in the intensity of skin reactions coincided with those observed in VDH responses and with lentinan-induced antitumor effects against Sarcoma 180. Augmentation of skin reactions was not observed in T-cell-deficient mice. Inhibitors of lipoxygenase, thrombin and plasmin which reduced skin reactions also decreased the incidence of tumor necrosis positive mice among FBL-3-bearing mice treated with lentinan. Furthermore, B10D2 mice treated with fluorouracl (5-FU) and lentinan 10 days after S908.D2 transplantation showed complete tumor regression and augmented skin reactions, whereas augmentation of skin reactions and tumor regression were not observed in mice treated with 5-FU and lentinan 32 days after tumor inoculation. Taken together, these results suggest that these vascular reactions might play crucial roles in antitumor effects of lentinan and that the skin reaction, the convenient method for investigating vascular reactions, is a promising tool to monitor host sensitivity to lentinan in antitumor responses.
在小鼠系统中研究了抗肿瘤多糖香菇多糖对血管活性介质的血管反应的影响。香菇多糖增强了对缓激肽的皮内反应。据报道，诱导急性期蛋白（APP）和耳部血管扩张出血（VDH）反应可反映宿主对香菇多糖的反应。皮肤反应强度的品系差异与VDH反应中观察到的差异以及香菇多糖对肉瘤180的抗肿瘤作用一致。在T细胞缺陷小鼠中未观察到皮肤反应增强。降低皮肤反应的脂氧合酶、凝血酶和纤溶酶抑制剂也降低了用香菇多糖处理的携带FBL-3小鼠中肿瘤坏死阳性小鼠的发生率。此外，在S908.D2移植后10天用氟尿嘧啶（5-FU）和香菇多糖处理的B10D2小鼠显示肿瘤完全消退且皮肤反应增强，而在肿瘤接种后32天用5-FU和香菇多糖处理的小鼠中未观察到皮肤反应增强和肿瘤消退。综上所述，这些结果表明这些血管反应可能在香菇多糖的抗肿瘤作用中起关键作用，并且皮肤反应作为研究血管反应的便捷方法，是监测宿主在抗肿瘤反应中对香菇多糖敏感性的有前途的工具。
