在感染HIV的同性恋男性精液中能否检测到卡波西肉瘤相关疱疹病毒？
Is Kaposi's-sarcoma-associated herpesvirus detectable in semen of HIV-infected homosexual men?
作者信息
Lin J C, Lin S C, Mar E C, Pellett P E, Stamey F R, Stewart J A, Spira T J
机构信息
Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
出版信息
Lancet. 1995 Dec 16;346(8990):1601-2. doi: 10.1016/s0140-6736(95)91931-7.
We explored a possible route of transmission of Kaposi's-sarcoma-associated herpes virus (KSHV) with nested and unnested PCR techniques. We looked for KSHV DNA sequences in semen of HIV-positive homosexual men and HIV-negative healthy semen donors. With unnested primers we found KSHV sequences in 21 of 33 (64%) homosexual men and in none of 30 healthy donors. With a nested PCR assay, 30 of 33 (91%) specimens from the homosexual men and 7 of 30 (23%) specimens from healthy donors had detectable KSHV sequences. Over 5 years of follow-up, 13 of 30 KSHV-positive homosexual men (43%) developed KS compared with none of the 3 KSHV-negative homosexual men.
我们运用巢式和非巢式聚合酶链反应（PCR）技术探索了卡波西肉瘤相关疱疹病毒（KSHV）的一种可能传播途径。我们在HIV阳性同性恋男性的精液以及HIV阴性健康精液捐献者的精液中寻找KSHV DNA序列。使用非巢式引物，我们在33名同性恋男性中的21名（64%）发现了KSHV序列，而在30名健康捐献者中均未发现。通过巢式PCR检测，33名同性恋男性的30份标本（91%）以及30名健康捐献者的7份标本（23%）检测到了可检测的KSHV序列。在超过5年的随访中，30名KSHV阳性同性恋男性中有13名（43%）发展为卡波西肉瘤，而3名KSHV阴性同性恋男性均未发病。
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