在感染HIV个体的精液中检测到人类疱疹病毒8型DNA，但在健康精液捐献者的精液中未检测到。
Detection of human herpesvirus 8 DNA in semen from HIV-infected individuals but not healthy semen donors.
作者信息
Howard M R, Whitby D, Bahadur G, Suggett F, Boshoff C, Tenant-Flowers M, Schulz T F, Kirk S, Matthews S, Weller I V, Tedder R S, Weiss R A
机构信息
Department of Virology, University College London Medical School, UK.
出版信息
AIDS. 1997 Feb;11(2):F15-9. doi: 10.1097/00002030-199702000-00001.
OBJECTIVE
To ascertain the prevalence of Kaposi's sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus (HHV) type 8, and cytomegalovirus (CMV) DNA in semen was investigated.
METHODS
Amplification by nested polymerase chain reaction was used to detect viral DNA sequences in samples from 24 HIV-infected gay men, 15 of them with Kaposi's sarcoma (KS), and 115 healthy donors.
RESULTS
Six of the 24 HIV-infected patients had detectable HHV-8 DNA in their semen: three of the 15 patients with KS and three of the nine patients without KS. CMV DNA was detected in 20 semen samples from HIV-infected patients. None of the semen samples from healthy donors had detectable HHV-8 DNA and rates of CMV DNA detection were low (3%).
CONCLUSIONS
The study demonstrates the presence of HHV-8 in semen from HIV-infected individuals with, or at risk, of developing KS and the potential for sexual transmission of the virus. We found no evidence of HHV-8 in the semen of HIV-uninfected donors.
目的
确定卡波西肉瘤相关疱疹病毒（KSHV，也称为人类疱疹病毒8型）和巨细胞病毒（CMV）在精液中的DNA流行情况。
方法
采用巢式聚合酶链反应进行扩增，以检测24名感染HIV的男同性恋者、其中15名患有卡波西肉瘤（KS）者以及115名健康供者的样本中的病毒DNA序列。
结果
24名感染HIV的患者中有6名精液中可检测到HHV-8 DNA：15名KS患者中有3名，9名无KS的患者中有3名。在20份感染HIV患者的精液样本中检测到CMV DNA。健康供者的精液样本中均未检测到可检测的HHV-8 DNA，CMV DNA检测率较低（3%）。
结论
该研究证明在感染HIV且有发生KS风险或已发生KS的个体的精液中存在HHV-8，以及该病毒存在性传播的可能性。我们在未感染HIV的供者精液中未发现HHV-8的证据。
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