Gatermann S G, Meyer H G
Institute for Medical Microbiology and Hygiene, University of Mainz, Germany.
Med Microbiol Immunol. 1995 Aug;184(2):81-5. doi: 10.1007/BF00221391.
Expression of two major surface proteins of Staphylococcus saprophyticus, the haemagglutinin and the Staphylococcus saprophyticus surface-associated protein (Ssp), required carefully defined culture conditions. The Ssp is produced when bacteria are grown on agar, whereas expression of the haemagglutinin requires growth in broth. We sought to identify the environmental signals that are responsible for this modulation. Varying the pH, the osmolarity of the growth medium or the temperature did not influence expression of the proteins. In contrast, growth in an anaerobic atmosphere increased haemagglutination titres and fibronectin binding (both mediated by the haemagglutinin) but suppressed production of the Ssp. As the influence of the CO2 level could be excluded, we conclude that expression of these surface proteins is probably modulated by the O2 content of the atmosphere.
腐生葡萄球菌两种主要表面蛋白（血凝素和腐生葡萄球菌表面相关蛋白[Ssp]）的表达需要严格定义的培养条件。当细菌在琼脂上生长时会产生Ssp，而血凝素的表达则需要在肉汤中生长。我们试图确定负责这种调节的环境信号。改变pH值、生长培养基的渗透压或温度均不影响这些蛋白的表达。相反，在厌氧环境中生长会增加血凝滴度和纤连蛋白结合（均由血凝素介导），但会抑制Ssp的产生。由于可以排除二氧化碳水平的影响，我们得出结论，这些表面蛋白的表达可能受大气中氧气含量的调节。
