Nixon A J
New Zealand Pastoral Agriculture Research Institute, Ruakura Agricultural Centre, Hamilton.
Biotech Histochem. 1993 Nov;68(6):316-25. doi: 10.3109/10520299309105637.
A histological method is described for determining the proportion of growing hair follicles in skin samples. A variation of the Sacpic staining method, modified for bulk processing, produces high contrast staining of the principal tissue types present in skin. In particular, the inner root sheath is accentuated, facilitating detection of active follicles. Skin preparations from a range of species are used to illustrate structural characteristics of follicles viewed in cross section at various stages of the hair cycle and to establish criteria for classification of the state of activity of follicles. The hair cycle may be divided into quiescent and active states at the points of rapid transition (early pronanagen and mid catagen). Data from repeated skin biopsies from ferrets and goats are also used to demonstrate quantitative estimation of follicle activity, change in compound follicle size, and the relationship between follicle type and fiber medullation.
本文描述了一种用于确定皮肤样本中生长毛囊比例的组织学方法。对Sacpic染色方法进行了改进，以适用于批量处理，该方法能对皮肤中主要组织类型进行高对比度染色。特别是，内根鞘被显著染色，便于检测活跃毛囊。使用来自一系列物种的皮肤标本，以说明在毛发周期不同阶段横切面上观察到的毛囊结构特征，并建立毛囊活性状态分类标准。在快速转变点（早期生长期和中期退行期），毛发周期可分为静止期和活跃期。来自雪貂和山羊的重复皮肤活检数据也用于证明毛囊活性的定量估计、复合毛囊大小的变化以及毛囊类型与纤维髓质化之间的关系。
