Estimation of the sizes of polymorphic C-bands in man by measurement of DNA content of whole chromosomes.
作者信息
Sumner A T
出版信息
Cytogenet Cell Genet. 1977;19(5):250-5. doi: 10.1159/000130817.
PMID:75083
Abstract
A method is proposed for estimating the sizes of polymorphic C-bands of human chromosomes by microdensitometric measurement of the DNA content of whole chromosomes. The method requires measurements of: a chromosome known not to by polymorphic, as a standard against which to normalize all measurements; the polymorphic chromosomes of interest; and a homologous polymorphic chromosome lacking the C-band. In an example studies here, the C-band of chromosome 9 was estimated to contain about 0.0296 pg of DNA.
摘要
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