Qu Z, Garcia C H, O'Rourke L M, Planck S R, Kohli M, Rosenbaum J T
Department of Cell Biology and Anatomy, Oregon Health Sciences University, Casey Eye Institute, Portland 97201.
Arthritis Rheum. 1994 Feb;37(2):212-20. doi: 10.1002/art.1780370210.
To test the hypothesis that local proliferation contributes significantly to the hyperplasia of rheumatoid synovium.
Immunohistologic and chemical staining was used to identify 3 markers of cell proliferation: proliferating cell nuclear antigen, c-myc proto-oncogene, and nucleolar organizer regions. Synovium from 21 patients with rheumatoid arthritis, 34 with degenerative joint disease, and 7 with joint trauma was examined.
All 3 markers indicated substantial, active proliferation of synovial lining cells in synovium with hyperplasia. Proliferating cells showed type I procollagen immunoreactivity but were negative for CD68, a monocyte/macrophage marker. Proliferation was greater in rheumatoid arthritis than in the other conditions evaluated.
In situ proliferation of fibroblast-like synoviocytes in the synovium lining contributes considerably to the increase in cell numbers in rheumatoid synovium.
检验局部增殖对类风湿性滑膜炎增生有显著贡献这一假说。
采用免疫组织化学和化学染色来鉴定细胞增殖的3种标志物：增殖细胞核抗原、c-myc原癌基因和核仁组织区。对21例类风湿性关节炎患者、34例退行性关节病患者和7例关节创伤患者的滑膜进行了检查。
所有3种标志物均表明增生滑膜中滑膜衬里细胞有大量活跃增殖。增殖细胞显示I型前胶原免疫反应性，但对单核细胞/巨噬细胞标志物CD68呈阴性。类风湿性关节炎中的增殖比其他评估情况更明显。
滑膜衬里中类成纤维细胞样滑膜细胞的原位增殖对类风湿性滑膜中细胞数量的增加有很大贡献。
