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猪鼻支原体变异脂蛋白VlpC的加工与表面呈递

Processing and surface presentation of the Mycoplasma hyorhinis variant lipoprotein VlpC.

作者信息

Cleavinger C M, Kim M F, Wise K S

机构信息

Department of Molecular Microbiology and Immunology, University of Missouri-Columbia 65212.

出版信息

J Bacteriol. 1994 Apr;176(8):2463-7. doi: 10.1128/jb.176.8.2463-2467.1994.

DOI:10.1128/jb.176.8.2463-2467.1994
PMID:7512554
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC205375/
Abstract

The variant surface lipoprotein VlpC of Mycoplasma hyorhinis was shown to be processed by cleavage of a characteristic prokaryotic prolipoprotein signal peptide. In addition, a vlpC::phoA fusion protein expressed and translocated in Escherichia coli was recognized by surface-binding monoclonal antibodies, which identified the characteristic region II of Vlps, containing divergent external sequences proximal to the membrane, as an exposed portion of these surface proteins subject to immune recognition and selection.

摘要

猪鼻支原体的可变表面脂蛋白VlpC经一种典型的原核前脂蛋白信号肽切割后进行加工。此外，在大肠杆菌中表达并转运的vlpC::phoA融合蛋白被表面结合单克隆抗体识别，该抗体将Vlp的特征性区域II鉴定为这些表面蛋白的一个暴露部分，该区域含有靠近膜的不同外部序列，易于受到免疫识别和选择。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10c1/205375/dce636c9e729/jbacter00026-0342-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10c1/205375/f87329b53924/jbacter00026-0340-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10c1/205375/bce223f9dbb2/jbacter00026-0341-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10c1/205375/dce636c9e729/jbacter00026-0342-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10c1/205375/f87329b53924/jbacter00026-0340-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10c1/205375/bce223f9dbb2/jbacter00026-0341-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/10c1/205375/dce636c9e729/jbacter00026-0342-a.jpg

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一种新型转座子构建体，表达 PhoA，可用于研究禽支原体蛋白表达和易位。
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Cloning of mnuA, a membrane nuclease gene of Mycoplasma pulmonis, and analysis of its expression in Escherichia coli.肺炎支原体膜核酸酶基因mnuA的克隆及其在大肠杆菌中的表达分析。
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Elongated versions of Vlp surface lipoproteins protect Mycoplasma hyorhinis escape variants from growth-inhibiting host antibodies.Vlp表面脂蛋白的延长版本可保护猪鼻支原体逃逸变体免受抑制生长的宿主抗体的影响。
Infect Immun. 1997 May;65(5):1773-85. doi: 10.1128/iai.65.5.1773-1785.1997.
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