Wong A K, Rattner J B
Department of Medical Biochemistry and Anatomy, University of Calgary, Alberta, Canada.
Nucleic Acids Res. 1988 Dec 23;16(24):11645-61. doi: 10.1093/nar/16.24.11645.
A complete 120 bp genomic consensus sequence for the mouse minor satellite has been determined from enriched L929 centromeric sequences. The extensive sequence homology existing between the major and minor satellite suggests an evolutionary relationship. Some sequences flanking the minor satellite has also been identified and they provide insight into centromeric DNA organization. Isotopic in situ hybridization analysis of the minor satellite to mouse L929 and Mus musculus metaphase spreads showed that this repetitive DNA class is localized specifically to centromeres of all chromosomes of the karyotype. With the use of high resolution non-isotopic fluorescence in situ hybridization the minor satellite is further localized to the outer surface of the centromere in a discrete region at or immediately adjacent to the kinetochore. Our cytological data suggests that the minor satellite might play a role in the organization of the kinetochore region rather than, as previously suggested, sites for general anchoring of the genome to the nuclear matrix.
从小鼠L929着丝粒序列富集物中已确定了小鼠小卫星的完整120bp基因组共有序列。主要卫星和小卫星之间存在的广泛序列同源性表明了一种进化关系。小卫星两侧的一些序列也已被鉴定出来，它们为着丝粒DNA组织提供了见解。小卫星与小鼠L929和小家鼠中期染色体铺片的同位素原位杂交分析表明，这种重复DNA类别特异性地定位于核型所有染色体的着丝粒。使用高分辨率非同位素荧光原位杂交，小卫星进一步定位于着丝粒的外表面，位于动粒处或紧邻动粒的一个离散区域。我们的细胞学数据表明，小卫星可能在动粒区域的组织中起作用，而不是像以前所认为的那样，是基因组与核基质一般锚定的位点。
