Scott S M, Barth M G, Gaddy L R, Ahl E T
Department of Surgery, Veterans Administration Medical Center, Asheville, NC 28805.
J Vasc Surg. 1994 Apr;19(4):585-93. doi: 10.1016/s0741-5214(94)70030-3.
Cells covering the flow surface of vascular prostheses are derived in part from endothelium of adjacent native artery and from capillaries that extend through the pores of the graft. This study is designed to determine whether these endothelial-like cells can also originate from circulating blood cells and if so to identify them with protein markers.
Pledgets of vascular graft material were suspended within the aortas of dogs with metal stents that isolated the pledgets from the aortic wall. Explanted pledgets were examined for cells containing factor VIII-related antigen and other markers identified with monoclonal antibodies.
A monolayer of cells that stained positive for factor VIII formed on pledgets after 7 days. Pledgets removed after 55 days had endothelial cells, smooth muscle cells, macrophages, monocytes, and capillary-like structures which were identified by staining for the monoclonal antibodies 43 beta E3 (vimentin), HHF35 (alpha and gamma muscle actin), CGA7 (smooth muscle actin), and HAM56 (macrophage). Woven and knitted polyester and extruded polytetrafluoroethylene pledgets healed in a similar manner.
The origin of the cells identified is speculative but they appear to have been derived from circulating cells, possibly stem cells, which are capable of differentiation because the pledgets on which the cells were identified were isolated from aortic wall endothelium and perivascular capillaries.
覆盖血管假体血流表面的细胞部分来源于相邻天然动脉的内皮以及延伸穿过移植物孔隙的毛细血管。本研究旨在确定这些内皮样细胞是否也能源自循环血细胞，如果可以，则用蛋白质标记物对它们进行识别。
将血管移植物材料的小片用金属支架悬浮在犬的主动脉内，该支架将小片与主动脉壁隔开。对取出的小片进行检查，寻找含有因子VIII相关抗原的细胞以及用单克隆抗体识别的其他标记物。
7天后，小片上形成了一层对因子VIII染色呈阳性的细胞单层。55天后取出的小片有内皮细胞、平滑肌细胞、巨噬细胞、单核细胞以及毛细血管样结构，通过对单克隆抗体43βE3（波形蛋白）、HHF35（α和γ肌动蛋白）、CGA7（平滑肌肌动蛋白）和HAM56（巨噬细胞）染色进行识别。编织和针织聚酯以及挤出聚四氟乙烯小片以类似方式愈合。
所识别细胞的来源具有推测性，但它们似乎源自循环细胞，可能是干细胞，这些细胞能够分化，因为识别出这些细胞的小片与主动脉壁内皮和血管周围毛细血管是隔开的。
