Burrows T D, King A, Loke Y W
Department of Pathology, University of Cambridge, United Kingdom.
Cell Immunol. 1993 Mar;147(1):81-94. doi: 10.1006/cimm.1993.1050.
This study has examined the expression of adhesion molecules by uterine-specific CD56bright CD16-CD3- large granular lymphocytes using flow cytometry and immunohistology. Data are compared with those obtained after IL-2 stimulation and with peripheral blood CD56+ cells. We have found that decidual CD56bright cells strongly express three fibronectin receptors, alpha 4 beta 1, alpha 5 beta 1, and alpha 4 beta 7. The HML-1 antigen is also expressed. The beta 2 integrins are also found on decidual CD56+ cells although, apart from CD11c, these are present at levels lower than those of corresponding cells in blood. After IL-2 stimulation there is a considerable increase in CD11a/CD18 expression on CD56bright cells. Both ICAM-1 and NCAM are found on CD56bright cells at higher levels compared to those of peripheral blood CD56+ cells. This pattern of expression of beta 1 and beta 2 integrins and members of the immunoglobulin superfamily must have important implications in the behaviour of decidual LGL. These adhesion molecules are also likely to be important in the recruitment and retention of CD56bright LGL in the decidual microenvironment particularly as the specialised decidual extracellular matrix and uterine LGL appear concomitantly at the time of implantation.
本研究利用流式细胞术和免疫组织学检测了子宫特异性CD56bright CD16-CD3-大颗粒淋巴细胞黏附分子的表达情况。将数据与白细胞介素-2刺激后获得的数据以及外周血CD56+细胞的数据进行了比较。我们发现，蜕膜CD56bright细胞强烈表达三种纤连蛋白受体，即α4β1、α5β1和α4β7。还表达了HML-1抗原。在蜕膜CD56+细胞上也发现了β2整合素，不过，除了CD11c外，这些整合素的表达水平低于血液中相应细胞的表达水平。白细胞介素-2刺激后，CD56bright细胞上的CD11a/CD18表达显著增加。与外周血CD56+细胞相比，CD56bright细胞上的细胞间黏附分子-1（ICAM-1）和神经细胞黏附分子（NCAM）的表达水平更高。β1和β2整合素以及免疫球蛋白超家族成员的这种表达模式必然对蜕膜大颗粒淋巴细胞的行为具有重要意义。这些黏附分子在蜕膜微环境中CD56bright大颗粒淋巴细胞的募集和滞留过程中可能也很重要，尤其是在着床时，特化的蜕膜细胞外基质和子宫大颗粒淋巴细胞同时出现。
