Stoianova L, Bineva I, Dilov P, Doneva M, Mermerski K
Vet Med Nauki. 1978;15(8):67-73.
Studies are the following preparations, produced by "PHARMACHIM": tylosine-base (Tylosine-50), tylosine tartarate (Tylosine pulvis) and tylosine phosphate (substance and granules) and their analogues: tylosine-base (Tylan-50), tylosine tartarate (Tylan soluble) and tylosine phosphate (Tylan AF 99), produced by the "Elanco" Company. The biological activity of the preparation was determined by means of the microbiological method of diffusion in agar. In the case of all experimental treatments, the preparations were compared in conformity with their biological activity expressed in IU/mg of the preparations. The minimum inhibition concentrations of the preparations were determined by the method of serial dilutions in liquid nutritive media on 19 different pathogenic strains. The antispirochetal activity of the preparations compared was tested on 130 cockerels and pullets, experimentally infected with Borrelia anserina of the Surnevo and Pamoukchii serotypes. The application of the preparations started simultaneously with the infection or 24 and 48 hrs after it and continued for days. No significant differences are found in the antibacterial and antispirochetal activities of the Bulgarian tylosine preparations and those manufactured by the "Elanko" Company. The tylosine base does not differ significantly in its antimicrobial and antispirochetal activities from the tylosine phosphate and the tylosine tartarate when equal in IU concentrations and doses are applied.
研究对象为“PHARMACHIM”公司生产的以下制剂：泰乐菌素碱（泰乐菌素 - 50）、泰乐菌素酒石酸盐（泰乐菌素粉剂）和泰乐菌素磷酸盐（原料药及颗粒剂）及其类似物：“Elanco”公司生产的泰乐菌素碱（泰兰 - 50）、泰乐菌素酒石酸盐（可溶性泰兰）和泰乐菌素磷酸盐（泰兰AF 99）。通过琼脂扩散微生物学方法测定制剂的生物活性。在所有实验处理中，根据制剂每毫克中以国际单位（IU）表示的生物活性对制剂进行比较。通过在液体营养培养基中对19种不同致病菌株进行连续稀释法测定制剂的最低抑菌浓度。在130只经苏尔内沃和帕穆基血清型的鹅疏螺旋体实验感染的公鸡和母鸡上测试所比较制剂的抗螺旋体活性。制剂在感染时或感染后24小时和48小时同时开始应用，并持续数天。保加利亚的泰乐菌素制剂与“Elanco”公司生产的制剂在抗菌和抗螺旋体活性方面未发现显著差异。当应用相同IU浓度和剂量时，泰乐菌素碱在抗菌和抗螺旋体活性方面与泰乐菌素磷酸盐和泰乐菌素酒石酸盐无显著差异。
