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Microbial transformation of immunosuppressive compounds. III. Glucosylation of immunomycin (FR 900520) and FK 506 by Bacillus subtilis ATCC 55060.

作者信息

Petuch B R, Arison B, Hsu A, Monaghan R, Dumont F J, Chen T S

机构信息

Merck Research Laboratories, Rahway, NJ 07065.

出版信息

J Ind Microbiol. 1994 Mar;13(2):131-5. doi: 10.1007/BF01584111.

DOI:10.1007/BF01584111
PMID:7513528
Abstract

The regiospecific glucosylation of FK 506 and immunomycin (FR 900520) at the 24-hydroxy position was performed using resting cells of Bacillus subtilis ATCC 55060. 24-Glucopyranosyl FK 506 and 24-glucopyranosyl immunomycin were isolated by methylene chloride extraction and purification using reverse phase HPLC. The metabolite structures were established using spectroscopic techniques including MS and NMR. The glucose conjugate was further confirmed by chemical degradation. Enzymatic glucosylation was demonstrated using cell-free extracts derived from Bacillus subtilis ATCC 55060. The 24-glucosyltransferase, which appears UDP-glucose dependent, was solubilized from cell membranes by treatment with 0.1% Nonidet P-40 detergent. The optimal conditions for assay of the enzyme have been determined.

摘要

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本文引用的文献

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Microbial transformation of immunosuppressive compounds. II. Specific desmethylation of 13-methoxy group of FK 506 and FR 900520 by an unidentified Actinomycete ATCC 53828 [corrected].免疫抑制化合物的微生物转化。II. 一种未鉴定的放线菌ATCC 53828对FK 506和FR 900520的13-甲氧基进行特异性去甲基化反应[校正后] 。
J Antibiot (Tokyo). 1992 Apr;45(4):577-80. doi: 10.7164/antibiotics.45.577.
9
Microbial transformation of immunosuppressive compounds. I. Desmethylation of FK506 and immunomycin (FR 900520) by Actinoplanes sp. ATCC 53771.
J Antibiot (Tokyo). 1992 Jan;45(1):118-23. doi: 10.7164/antibiotics.45.118.
10
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.一种利用蛋白质 - 染料结合原理对微克级蛋白质进行定量的快速灵敏方法。
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