Cai H Y
Cancer Center, Sun Yet-sen University of Medical Sciences, Guangzhou, Guangdong.
Zhonghua Zhong Liu Za Zhi. 1993 Jul;15(4):248-51.
We have successfully established two human esophageal carcinoma models in nude mice by transplantation of surgical specimen from patients with esophageal cancer. Histopathological study revealed that one was squamous cell carcinoma (HEC2) and the other adeno-squamous cell carcinoma (HEC6). Light and electron microscopy showed that their morphology was similar to that of the original tumors. The transplanted tumors have been maintained for more than four years through more than 50 passages. The tumors grew steadily and fast. The rate of successful inoculation was 100%. Bleomycin A5 and 5-Fluorouracil (5-Fu) were used for treatment of HEC6. Results showed that Bleomycin A5 exhibited a remarkable growth inhibition on the HEC6. The inhibition rates of two separate experiments were 56.5% and 87.8% respectively (compared with the control group, P < 0.01). The inhibition rates of 5-Fu were 6.5% and 36.6% respectively. Compared with the control group, there was no significant difference statistically HEC2 was treated by X-ray or X-ray combined with hematoporphyrin derivative (HPD), the tumor growth inhibition was 57.7% and 85.4% respectively.
我们通过移植食管癌患者的手术标本，成功在裸鼠体内建立了两种人食管癌模型。组织病理学研究显示，一种是鳞状细胞癌（HEC2），另一种是腺鳞癌（HEC6）。光镜和电镜检查表明，它们的形态与原发肿瘤相似。移植瘤已传代50多次，维持了四年多。肿瘤生长稳定且迅速。接种成功率为100%。用博来霉素A5和5-氟尿嘧啶（5-Fu）治疗HEC6。结果显示，博来霉素A5对HEC6有显著的生长抑制作用。两个独立实验的抑制率分别为56.5%和87.8%（与对照组相比，P<0.01）。5-Fu的抑制率分别为6.5%和36.6%。与对照组相比，差异无统计学意义。HEC2用X射线或X射线联合血卟啉衍生物（HPD）治疗，肿瘤生长抑制率分别为57.7%和85.4%。
