Araki A, Noguchi T, Kato F, Matsushima T
Department of Microbial Mutagenicity Test, Japan Bioassay Laboratory, Industrial Safety and Health Association, Kanagawa, Japan.
Mutat Res. 1994 May 1;307(1):335-44. doi: 10.1016/0027-5107(94)90307-7.
A simple and safety gas exposure method was developed using a gas sampling bag as an exposure vessel and a preparation vessel of diluted gas. The gas exposure conditions such as amount of S9 in the plate, volume of gas for the plate, amount of top agar, exposure period and exposure temperature were examined by mutagenicity testing of 1,3-butadiene using the gas sampling bag. Mutagenicity tests of 14 compounds and 1,3-butadiene on S. typhimurium TA98, TA100, TA1535 and TA1537, and E. coli WP2 uvrA were also examined by the developed gas exposure method. 1,3-Butadiene, propyne (methyl acetylene), monochlorodifluoromethane, ethylchloride, diborane and silane were mutagenic. 1-Butene, 2-butene, 2-methylpropene, methyl vinyl ether, trichlorofluoromethane, dichlorodifluoromethane, 1,2-dichloro-1,1,2,2-tetrafluoroethane, 1,1-difluoroethane and phosphine were not mutagenic on S. typhimurium TA98, TA100, TA1535 and TA1537, and E. coli WP2 uvrA with or without metabolic activation. These results were compatible with a previous report, and this developed method has the advantage that it can be tested easily and safely for combustible and self-combustible substances such as 1,3-butadiene and silane.
开发了一种简单且安全的气体暴露方法，该方法使用气体采样袋作为暴露容器和稀释气体的制备容器。通过使用气体采样袋对1,3 - 丁二烯进行致突变性测试，考察了平板中S9的量、平板的气体体积、顶层琼脂的量、暴露时间和暴露温度等气体暴露条件。还通过开发的气体暴露方法对14种化合物和1,3 - 丁二烯在鼠伤寒沙门氏菌TA98、TA100、TA1535和TA1537以及大肠杆菌WP2 uvrA上进行了致突变性测试。1,3 - 丁二烯、丙炔（甲基乙炔）、一氯二氟甲烷、氯乙烷、乙硼烷和硅烷具有致突变性。1 - 丁烯、2 - 丁烯、2 - 甲基丙烯、甲基乙烯基醚、三氯氟甲烷、二氯二氟甲烷、1,2 - 二氯 - 1,1,2,2 - 四氟乙烷、1,1 - 二氟乙烷和磷化氢在有或没有代谢活化的情况下，对鼠伤寒沙门氏菌TA98、TA100、TA1535和TA1537以及大肠杆菌WP2 uvrA均无致突变性。这些结果与先前的报告一致，并且这种开发的方法具有可以轻松、安全地对1,3 - 丁二烯和硅烷等可燃和自燃物质进行测试的优点。
